Evidence map›Paper›PMID 31690619›Full record

ArticleThe Journal of cell biology2019

Confinement hinders motility by inducing RhoA-mediated nuclear influx, volume expansion, and blebbing.

Panagiotis Mistriotis, Emily O Wisniewski, Kaustav Bera, Jeremy Keys, Yizeng Li, Soontorn Tuntithavornwat, Robert A Law, Nicolas A Perez-Gonzalez, Eda Erdogmus, Yuqi Zhang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed.

  1. Review
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  12. Chemical compensation to mechanical loss in cell mechanosensation.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
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  14. Review
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21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Panagiotis Mistriotis *Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.ORCID 0000-0002-8069-3278
Emily O Wisniewski *Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Kaustav BeraDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Jeremy KeysMeinig School of Biomedical Engineering and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY.
Yizeng LiJohns Hopkins Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD.
Soontorn TuntithavornwatDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Robert A LawDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Nicolas A Perez-GonzalezDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Eda ErdogmusDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Yuqi ZhangDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.
Runchen ZhaoDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.ORCID 0000-0001-8673-3990
Sean X SunJohns Hopkins Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD.ORCID 0000-0002-9077-7088
Petr KalabDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD.ORCID 0000-0002-8145-7728
Jan LammerdingMeinig School of Biomedical Engineering and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY.ORCID 0000-0003-4335-8611
Konstantinos KonstantopoulosDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD konstant@jhu.edu.ORCID 0000-0003-2623-1459

Funding

Project 3: Physical and Metabolic Constraints of Cancer Cell InvasionU54CA210184 · NCI · CORNELL UNIVERSITY · PI SCHAFFER, CHRIS B · 2016 to 2020
$10.2M
The Role of Physical Cues in Collective Cell InvasionU54CA210173 · NCI · JOHNS HOPKINS UNIVERSITY · PI PIENTA, KENNETH J., WIRTZ, DENIS · 2016 to 2020
$9.9M
Nuclear mechanics and mechanotransduction in muscular laminopathiesR01HL082792 · NHLBI · CORNELL UNIVERSITY · PI LAMMERDING, JAN · 2007 to 2023
$4.8M
T32: Predoctoral and Postdoctoral Training Program in Nanotechnology for Cancer ResearchT32CA153952 · NCI · JOHNS HOPKINS UNIVERSITY · PI Denis Wirtz · 2015 to 2026
$3.5M
Development of high throughput screening technologies in breast cancerR01CA183804 · NCI · JOHNS HOPKINS UNIVERSITY · PI KONSTANTOPOULOS, KONSTANTINOS, KONTROGIANNI-KONSTANTOPOULOS, AIKATERINI · 2015 to 2019
$3.5M
T-32 NIH Postdoctoral Training Program in Nanotechnology for Cancer Medicine NTCMT32CA130840 · NCI · JOHNS HOPKINS UNIVERSITY · PI WIRTZ, DENIS · 2008 to 2012
$1.2M
Broad wavelength range Zeiss 780 NLO/confocal system for the Cornell Imaging CoreS10OD018516 · OD · CORNELL UNIVERSITY · PI ZIPFEL, WARREN R · 2014 to 2014
$834k
Acquisition of a PicoQuant FLIM SystemS10OD020007 · OD · JOHNS HOPKINS UNIVERSITY · PI MCCAFFERY, J. MICHAEL · 2016 to 2016
$231k
American Heart Association-American Stroke Association 18POST34080345NCI NIH HHS R01 CA183804NCI NIH HHS T32 CA130840NCI NIH HHS T32 CA153952NCI NIH HHS U54 CA210173NCI NIH HHS U54 CA210184NHLBI NIH HHS R01 HL082792NIH HHS S10 OD018516NIH HHS S10 OD020007
6 · The paper itself

Abstract

Cells migrate in vivo through complex confining microenvironments, which induce significant nuclear deformation that may lead to nuclear blebbing and nuclear envelope rupture. While actomyosin contractility has been implicated in regulating nuclear envelope integrity, the exact mechanism remains unknown. Here, we argue that confinement-induced activation of RhoA/myosin-II contractility, coupled with LINC complex-dependent nuclear anchoring at the cell posterior, locally increases cytoplasmic pressure and promotes passive influx of cytoplasmic constituents into the nucleus without altering nuclear efflux. Elevated nuclear influx is accompanied by nuclear volume expansion, blebbing, and rupture, ultimately resulting in reduced cell motility. Moreover, inhibition of nuclear efflux is sufficient to increase nuclear volume and blebbing on two-dimensional surfaces, and acts synergistically with RhoA/myosin-II contractility to further augment blebbing in confinement. Cumulatively, confinement regulates nuclear size, nuclear integrity, and cell motility by perturbing nuclear flux homeostasis via a RhoA-dependent pathway.

Indexed as

Actin CytoskeletonActinsActomyosinCell Line, TumorCell MovementCell NucleusCytoplasmFluorescence Resonance Energy TransferHomeostasisHumansMyosin Type IINuclear EnveloperhoA GTP-Binding ProteinTumor MicroenvironmentActinsActomyosinMyosin Type IIrhoA GTP-Binding ProteinRHOA protein, human

Identifiers

PMID31690619
PMCPMC6891075

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.