ArticleThe EMBO journal1988
Identification of domains involved in nuclear uptake and histone binding of protein N1 of Xenopus laevis.
Article in The EMBO journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Evidence for the nuclear import of histones H3.1 and H4 as monomers.The EMBO journal · 2018Article
- Identification of a nuclear localization sequence in β-arrestin-1 and its functional implications.The Journal of biological chemistry · 2012Article
- The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms.Nucleic acids research · 2012Article
- Cancer nanotheranostics: improving imaging and therapy by targeted delivery across biological barriers.Advanced materials (Deerfield Beach, Fla.) · 2011Review
- Depletion of the histone chaperone tNASP inhibits proliferation and induces apoptosis in prostate cancer PC-3 cells.Reproductive biology and endocrinology : RB&E · 2011Article
- Engineered biological entities for drug delivery and gene therapy protein nanoparticles.Progress in molecular biology and translational science · 2011Review
- Article
- Expanded binding specificity of the human histone chaperone NASP.Nucleic acids research · 2008Article
- sNASP, a histone H1-specific eukaryotic chaperone dimer that facilitates chromatin assembly.Biophysical journal · 2008Article
- A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres.Molecular cell · 2007Article
- Peptide-guided gene delivery.The AAPS journal · 2007Review
- Identification of a structural and functional domain in xNAP1 involved in protein-protein interactions.Nucleic acids research · 2006Article
- Mapping Hsp47 binding site(s) using CNBr peptides derived from type I and type II collagen.Protein science : a publication of the Protein Society · 2003Article
- Properties of the protein kinase that phosphorylates prothymosin alpha.Molecular and cellular biochemistry · 2000Article
- Further characterization of the actin-related protein Act3p/Arp4 of S. cerevisiae through mutational analysis.Molecular biology reports · 2000Article
- Analysis of a nuclear localization signal of simian virus 40 major capsid protein Vp1.Journal of virology · 1996Article
- Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean.Molecular & general genetics : MGG · 1995Article
- Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport.Journal of virology · 1995Article
- Human foamy virus Bel1 transactivator contains a bipartite nuclear localization determinant which is sensitive to protein context and triple multimerization domains.Journal of virology · 1995Article
- The U1 small nuclear ribonucleoprotein (snRNP) 70K protein is transported independently of U1 snRNP particles via a nuclear localization signal in the RNA-binding domain.Molecular and cellular biology · 1994Article
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2 authors.
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Abstract
The karyophilic protein N1 (590 amino acids) is an abundant soluble protein of the nuclei of Xenopus laevis oocytes where it forms defined complexes with histones H3 and H4. The amino acid sequence of this protein, as deduced from the cDNA, reveals a putative nuclear targeting signal as well as two acidic domains which are candidates for the interaction with histones. Using two different histone binding assays in vitro we have found that the deletion of the larger acidic domain reduces histone binding drastically to a residual value of approximately 15% of the complete molecule, whereas removal of the smaller acidic domain only slightly reduces histone complex formation in solution, but infers more effectively with binding to immobilized histones. In the primary structure of the protein both histone-binding domains are distant from the conspicuous nuclear accumulation signal sequence (residues 531-537) close to the carboxy terminus which is very similar to the SV40 large T-antigen nuclear targeting sequence. Using a series of N1 mutants altered by deletions or point mutations we show that this signal is required but not sufficient for nuclear accumulation of protein N1. The presence of an additional, more distantly related signal sequence in position 544-554 is also needed to achieve a level of nuclear uptake equivalent to that of the wild-type protein. Results obtained with point mutations support the concept of two nuclear targeting sequences and emphasize the importance of specific lysine and arginine residues in these signal sequences.
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