ArticleRegenerative therapy2019
Proliferation medium in three-dimensional culture of auricular chondrocytes promotes effective cartilage regeneration
Article in Regenerative therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- A proof-of-concept study of an albumin-based bilayered scaffold for cartilage regeneration.Materials today. Bio · 2026Article
- Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway.Regenerative therapy · 2026Article
- Innovative tissue engineering strategies for auricular regeneration in microtia: Current advances and future perspectives.Regenerative therapy · 2025Review
- Cartilage Laser Engraving for Fast-Track Tissue Engineering of Auricular Grafts.International journal of molecular sciences · 2024Article
- Impact of local delivery of allogeneic chondrocytes on the biological response and healing of the sternum bones after sternotomy.Scientific reports · 2023Article
- Article
- Additive Manufacturing of Polyhydroxyalkanoate-Based Blends Using Fused Deposition Modelling for the Development of Biomedical Devices.Journal of functional biomaterials · 2023Article
- Effect of Collagen and GelMA on Preservation of the Costal Chondrocytes' Phenotype in a ScaffoldSovremennye tekhnologii v meditsine · 2023Article
- Strategies to Convert Cells into Hyaline Cartilage: Magic Spells for Adult Stem Cells.International journal of molecular sciences · 2022Review
- Regeneration of Articular Cartilage Using Membranes of Polyester Scaffolds in a Rabbit Model.Pharmaceutics · 2022Article
- Biochemical Aspects of Scaffolds for Cartilage Tissue Engineering; from Basic Science to Regenerative Medicine.The archives of bone and joint surgery · 2022Review
- Restoration of the Phenotype of Dedifferentiated Rabbit Chondrocytes by Sesquiterpene Farnesol.Pharmaceutics · 2022Article
- Bioprinting of Cartilage with Bioink Based on High-Concentration Collagen and Chondrocytes.International journal of molecular sciences · 2021Article
- Tuning the Properties of PNIPAm-Based Hydrogel Scaffolds for Cartilage Tissue Engineering.Polymers · 2021Review
- Different phenotypes and chondrogenic responses of human menstrual blood and bone marrow mesenchymal stem cells to activin A and TGF-β3.Stem cell research & therapy · 2021Article
- Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications.Biomedicines · 2021Article
- Effects of nanopatterned-surface dishes on chondrocyte growth and cell cycle progression.RSC advances · 2020Article
- The usefulness of the decellularized matrix from three-dimensional regenerative cartilage as a scaffold material.Regenerative therapy · 2020Article
- Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionCartilage regeneration have been attracted attentions because of the poor ability of cartilage tissues to regenerate. Three-dimensional (3D) culture of chondrocytes is considered to be advantageous for cartilage regeneration. Although it is plausible that maturation of the constructs before transplantation positively affects the chondrogenesis, matured constructs after cultures for longer periods do not necessarily result in effective cartilage regeneration. In this study, we compared different types of culture media including growth factors which are clinically available. We prepared differentiation medium containing insulin-like growth factor-1 (IGF-1), proliferation medium containing fibroblast growth factor-2 (FGF-2) and insulin, and combination of them, and compared their efficacies on chondrogenesis when used in 3D culture of engineered cartilage constructs.
methodsCartilage constructs were fabricated by auricular chondrocytes and atelocollagen, and they were 3D-cultured with four types of media: control medium, differentiation medium, proliferation medium, and combination medium. After 3 weeks of culture, the constructs were analyzed for cell number, gene and protein expressions and mechanical properties. The constructs were also transplanted into nude mice. After 8 weeks, the degree of cartilage regeneration was evaluated. Constructs manufactured with canine auricular chondrocytes were subjected to autologous transplantation into beagles and examined for cartilage regeneration.
resultsDuring 3D culture, remarkably high gene expression of type II collagen was detected in the construct cultured with the differentiation medium whereas cell apoptosis were suppressed in the proliferation medium. When transplanted into nude mice, the constructs 3D-cultured in the proliferation medium produced abundant cartilage matrices. In autologous implantation model, the construct cultured in the proliferation medium again showed better chondrogenesis than those in other media.
conclusionsThe present study indicates that 3D culture with the proliferation medium maintains the cell viability to potentiate the subsequent cartilage regeneration
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