Evidence map›Paper›PMID 31685841›Full record

ArticleScientific reports2019

Localized delivery of compounds into articular cartilage by using high-intensity focused ultrasound.

Heikki J Nieminen, Eetu Lampsijärvi, Gonçalo Barreto, Mikko A J Finnilä, Ari Salmi, Anu J Airaksinen, Kari K Eklund, Simo Saarakkala, Kenneth P H Pritzker, Edward Hæggström

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Heikki J NieminenElectronics Research Laboratory, Department of Physics, University of Helsinki, Helsinki, Finland. heikki.j.nieminen@aalto.fi.
Eetu LampsijärviElectronics Research Laboratory, Department of Physics, University of Helsinki, Helsinki, Finland.
Gonçalo BarretoDepartment of Medicine, University of Helsinki, Helsinki, Finland.
Mikko A J FinniläResearch Group of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland.ORCID http://orcid.org/0000-0002-3348-5759
Ari SalmiElectronics Research Laboratory, Department of Physics, University of Helsinki, Helsinki, Finland.ORCID http://orcid.org/0000-0001-8768-1205
Anu J AiraksinenDepartment of Chemistry - Radiochemistry, University of Helsinki, Helsinki, Finland.
Kari K EklundDepartment of Rheumatology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.ORCID http://orcid.org/0000-0002-5870-4663
Simo SaarakkalaResearch Group of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland.ORCID http://orcid.org/0000-0003-2850-5484
Kenneth P H PritzkerDepartment of Laboratory Medicine and Pathobiology, University of Toronto and Mount Sinai Hospital, Toronto, Canada.
Edward HæggströmElectronics Research Laboratory, Department of Physics, University of Helsinki, Helsinki, Finland.
University of Helsinki · FIMount Sinai Hospital · CAOulu University Hospital · FIUniversity of Oulu · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Localized delivery of drugs into an osteoarthritic cartilaginous lesion does not yet exist, which limits pharmaceutical management of osteoarthritis (OA). High-intensity focused ultrasound (HIFU) provides a means to actuate matter from a distance in a non-destructive way. In this study, we aimed to deliver methylene blue locally into bovine articular cartilage in vitro. HIFU-treated samples (n = 10) were immersed in a methylene blue (MB) solution during sonication (f = 2.16 MHz, peak-positive-pressure = 3.5 MPa, mechanical index = 1.8, pulse repetition frequency = 3.0 kHz, cycles per burst: 50, duty cycle: 7%). Adjacent control 1 tissue (n = 10) was first pre-treated with HIFU followed by immersion into MB; adjacent control 2 tissue (n = 10) was immersed in MB without ultrasound exposure. The MB content was higher (p < 0.05) in HIFU-treated samples all the way to a depth of 600 µm from AC surface when compared to controls. Chondrocyte viability and RNA expression levels associated with cartilage degeneration were not different in HIFU-treated samples when compared to controls (p > 0.05). To conclude, HIFU delivers molecules into articular cartilage without major short-term concerns about safety. The method is a candidate for a future approach for managing OA.

Indexed as

High-Intensity Focused Ultrasound AblationAnimalsCartilage, ArticularCattleCell SurvivalChondrocytesMatrix MetalloproteinasesMethylene BlueOsteoarthritisTransforming Growth Factor betaMatrix MetalloproteinasesMethylene BlueTransforming Growth Factor beta

Identifiers

PMID31685841
PMCPMC6828772
OpenAlexW2987956091

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.