ArticleNature communications2019
Membrane protein megahertz crystallography at the European XFEL.
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Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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27citing papers in PubMed
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27 citing papers in PubMed, 63 citations in OpenAlex.
- High-resolution structure of monomeric photosystem I from Thermosynechococcus vestitus.Photosynthesis research · 2026Article
- High-Speed Detection of X-ray Pulses Using a Digital Counter Coupled with Perovskite Composite Scintillators.ACS applied materials & interfaces · 2026Article
- PEO-sheathed liquid jets increase sample delivery stability for serial femtosecond X-ray crystallography.Scientific reports · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Detergent Choice Shapes the Solution Structures of Photosystems I and II: Implications for Crystallization and High-Resolution Studies.The journal of physical chemistry. B · 2025Article
- Article
- Development of a TSR-based method for understanding structural relationships of cofactors and local environments in photosystem I.BMC bioinformatics · 2025Article
- How the Electron-Transfer Cascade is Maintained in Chlorophyll-Biochemistry · 2025Article
- Impact of Peripheral Hydrogen Bond on Electronic Properties of the Primary Acceptor Chlorophyll in the Reaction Center of Photosystem I.International journal of molecular sciences · 2024Article
- Recent Progress in Solution Structure Studies of Photosynthetic Proteins Using Small-Angle Scattering Methods.Molecules (Basel, Switzerland) · 2023Review
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.Lab on a chip · 2023Article
- Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics.Annual review of biophysics · 2023Review
- Molecular size dependence on achievable resolution from XFEL single-particle 3D reconstruction.Structural dynamics (Melville, N.Y.) · 2023Article
- Absolute quantification of cellular levels of photosynthesis-related proteins in Synechocystis sp. PCC 6803.Photosynthesis research · 2023Article
- An experimental study of liquid micro-jets produced with a gas dynamic virtual nozzle under the influence of an electric field.Frontiers in molecular biosciences · 2023Article
- Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.Nature communications · 2022Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Review
- Phase Diversity Electro-optic Sampling: A new approach to single-shot terahertz waveform recording.Light, science & applications · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
78 authors at 15 institutions in 5 countries.
Chris GisrielBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.ORCID http://orcid.org/0000-0002-8011-0052
Jesse CoeBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Romain LetrunEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID http://orcid.org/0000-0002-0569-5193
Oleksandr M YefanovCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Cesar Luna-ChavezBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.ORCID http://orcid.org/0000-0002-4214-9714
Natasha E StanderBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Stella LisovaBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Valerio MarianiCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Manuela KuhnCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Steve AplinCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Thomas D GrantHauptman-Woodward Institute, 700 Ellicott St, Buffalo, NY, 14203-1102, USA.ORCID http://orcid.org/0000-0002-8583-3700
Katerina DörnerEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Tokushi SatoEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Austin EchelmeierBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.ORCID http://orcid.org/0000-0001-6127-3879
Jorvani Cruz VillarrealBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.ORCID http://orcid.org/0000-0002-6762-7348
Mark S HunterLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, 94025, CA, USA.
Max O WiedornCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Juraj KnoskaCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Victoria MazalovaCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.ORCID http://orcid.org/0000-0003-2613-7868
Shatabdi Roy-ChowdhuryBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Jay-How YangBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Alex JonesBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Richard BeanEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Johan BieleckiEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Yoonhee KimEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Grant MillsEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Britta WeinhausenEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Jose D MezaEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Nasser Al-QudamiEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Saša BajtDeutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.ORCID http://orcid.org/0000-0002-7163-7602
Gerrit BrehmBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Sabine BothaDepartment of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA.
Djelloul BoukhelefEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Sandor BrockhauserEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID http://orcid.org/0000-0002-9700-4803
Barry D BruceDepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee at Knoxville, Knoxville, TN, USA, 37996.ORCID http://orcid.org/0000-0002-4045-9815
Matthew A ColemanLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
Cyril DanilevskiEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Erin DisciannoBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Zachary DobsonBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Hans FangohrEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID http://orcid.org/0000-0001-5494-7193
Jose M Martin-GarciaBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Yaroslav GevorkovCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.ORCID http://orcid.org/0000-0001-9273-7648
Steffen HaufEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Ahmad HosseinizadehDepartment of Physics, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.
Friederike JanuschekEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Gihan K KetawalaBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Christopher KupitzLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, 94025, CA, USA.
Luis MaiaEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Maurizio ManettiEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Marc MesserschmidtBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Thomas MichelatEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID http://orcid.org/0000-0002-5689-8759
Jyotirmoy MondalDepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee at Knoxville, Knoxville, TN, USA, 37996.ORCID http://orcid.org/0000-0002-0829-220X
Abbas OurmazdDepartment of Physics, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.ORCID http://orcid.org/0000-0001-9946-3889
Gianpietro PrevitaliEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Iosifina SarrouCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Silvan SchönCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Peter SchwanderDepartment of Physics, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.
Megan L ShelbyLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
Alessandro SilenziEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Jolanta Sztuk-DambietzEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Janusz SzubaEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Monica TurcatoEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.ORCID http://orcid.org/0000-0002-7928-5752
Thomas A WhiteCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.ORCID http://orcid.org/0000-0002-8407-0270
Krzysztof WronaEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Chen XuEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Mohamed H AbdellatifCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
James D ZookBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
John C H SpenceBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Henry N ChapmanCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.ORCID http://orcid.org/0000-0002-4655-1743
Anton BartyCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Richard A KirianBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Matthias FrankLawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.ORCID http://orcid.org/0000-0002-5244-0791
Alexandra RosBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.
Marius SchmidtDepartment of Physics, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, WI, 53211, USA.ORCID http://orcid.org/0000-0002-0962-9468
Raimund FrommeBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA.ORCID http://orcid.org/0000-0003-4835-1080
Adrian P MancusoEuropean XFEL GmbH, Holzkoppel 4, 22869, Schenefeld, Germany.
Petra FrommeBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA. pfromme@asu.edu.
Nadia A ZatsepinBiodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ, 85287-5001, USA. N.Zatsepin@latrobe.edu.au.
European X-Ray Free-Electron Laser · DEArizona State University · USUniversität Hamburg · DEUniversity of Wisconsin–Milwaukee · USLawrence Livermore National Laboratory · USDeutsches Elektronen-Synchrotron DESY · DELa Trobe University · AUSLAC National Accelerator Laboratory · USHauptman-Woodward Medical Research Institute · USHUN-REN Szegedi Biológiai Kutatóközpont · HUKnoxville College · USNanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103 · DEUniversity of Southampton · GBUniversity of Tennessee at Knoxville · USYale University · US
Funding
Technology development for biological imaging with x-ray free electron lasersR01GM117342 · NIGMS · LAWRENCE LIVERMORE NATIONAL SECURITY, LLC · PI FRANK, MATTHIAS · 2016 to 2024
$4.8MFemtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3MNIGMS NIH HHS R01 GM095583NIGMS NIH HHS R01 GM117342
Abstract
The world's first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 Å-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.
Indexed as
ElectronsLasersCrystallographyCyanobacteriaMembrane ProteinsModels, MolecularPhotosystem I Protein ComplexStatic ElectricitySynchrotronsThermosynechococcusX-RaysMembrane ProteinsPhotosystem I Protein Complex
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