Evidence map›Paper›PMID 31685521›Full record

ArticleGenetics2020

Gene Knock-Ins in

Justin A Bosch, Ryan Colbeth, Jonathan Zirin, Norbert Perrimon

Open access · bronzeAbstract read
In one paragraph

Article in Genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Expanding theeLife · 2024
    Article
  9. Article
  10. Article
  11. Article
  12. CRISPR/Cas9-mediated knock-in inGene and genome editing · 2023
    Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Demonstration ofbioRxiv : the preprint server for biology · 2023
    Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Justin A BoschDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115.ORCID 0000-0001-8499-1566
Ryan ColbethDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115.
Jonathan ZirinDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115.
Norbert PerrimonDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115 perrimon@genetics.med.harvard.edu.ORCID 0000-0001-7542-472X
Harvard University · USBoston VA Research Institute · US

Funding

New collections of dsRNAs and overexpression miRNA constructs to supplement RNAiR01GM067761 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI PERRIMON, NORBERT · 2003 to 2019
$15.5M
Drosophila Transgenic RNAi Resource ProjectR01GM084947 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2008 to 2019
$9.8M
Functional genomics resources for the Drosophila - TR&D3P41GM132087 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2019 to 2023
$6.8M
Next-generation Drosophila cell lines to elucidate the cellular basis of human diseasesR24OD019847 · OD · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT, SIMCOX, AMANDA · 2017 to 2020
$2.7M
Howard Hughes Medical InstituteNIGMS NIH HHS P41 GM132087NIGMS NIH HHS R01 GM067761NIGMS NIH HHS R01 GM084947NIH HHS R24 OD019847
6 · The paper itself

Abstract

Targeted genomic knock-ins are a valuable tool to probe gene function. However, knock-in methods involving homology-directed repair (HDR) can be laborious. Here, we adapt the mammalian CRISPaint [clustered regularly interspaced short palindromic repeat (CRISPR)-assisted insertion tagging] homology-independent knock-in method for

Indexed as

Amino Acid SequenceAnimalsBase SequenceCRISPR-Cas SystemsDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianGene Knock-In TechniquesGenomeMaleMutagenesis, InsertionalPlasmidsDrosophila ProteinsCRISPaintCRISPR/Cas9gene functionhomology-independentknock-in

Identifiers

PMID31685521
PMCPMC6944404
OpenAlexW2986502034

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.