ArticleOptics express2019
Efficient non-degenerate two-photon excitation for fluorescence microscopy.
Article in Optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Bessel beam side lobe suppression via non-degenerate two-photon excitation.Biomedical optics express · 2026Article
- "There's plenty of room at the bottom": deep brain imaging with holographic endo-microscopy.Neurophotonics · 2024Article
- Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.Biomedical optics express · 2024Article
- Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window.bioRxiv : the preprint server for biology · 2024Article
- Structural and photophysical characterization of the small ultra-red fluorescent protein.Nature communications · 2023Article
- Three-photon excited fluorescence imaging in neuroscience: From principles to applications.Frontiers in neuroscience · 2023Review
- Baseline oxygen consumption decreases with cortical depth.PLoS biology · 2022Article
- Diamond Raman laser and Yb fiber amplifier forBiomedical optics express · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain.Science advances · 2021Article
- Comparing the fundamental imaging depth limit of two-photon, three-photon, and non-degenerate two-photon microscopy.Optics letters · 2020Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Non-degenerate two-photon excitation (ND-TPE) has been explored in two-photon excitation microscopy. However, a systematic study of the efficiency of ND-TPE to guide the selection of fluorophore excitation wavelengths is missing. We measured the relative non-degenerate two-photon absorption cross-section (ND-TPACS) of several commonly used fluorophores (two fluorescent proteins and three small-molecule dyes) and generated 2-dimensional ND-TPACS spectra. We observed that the shape of a ND-TPACS spectrum follows that of the corresponding degenerate two-photon absorption cross-section (D-TPACS) spectrum, but is higher in magnitude. We found that the observed enhancements are higher than theoretical predictions.
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Registered trials
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