Evidence map›Paper›PMID 31683893›Full record

ArticleViruses2019

No Evidence of Mosquito Involvement in the Transmission of Equine Hepacivirus (Flaviviridae) in an Epidemiological Survey of Austrian Horses.

Marcha Badenhorst, Phebe de Heus, Angelika Auer, Till Rümenapf, Birthe Tegtmeyer, Jolanta Kolodziejek, Norbert Nowotny, Eike Steinmann, Jessika-M V Cavalleri

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Marcha BadenhorstUniversity Equine Clinic - Internal Medicine, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. marcha.badenhorst@vetmeduni.ac.at.
Phebe de HeusUniversity Equine Clinic - Internal Medicine, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. phebe.de-heus@vetmeduni.ac.at.ORCID 0000-0002-3799-2096
Angelika AuerInstitute of Virology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. angelika.auer@vetmeduni.ac.at.
Till RümenapfInstitute of Virology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. till.ruemenapf@vetmeduni.ac.at.
Birthe TegtmeyerInstitute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research, Medical School Hannover (MHH) - Helmholtz Centre for Infection Research (HZI), Feodor-Lynen-Strasse 7, 30625 Hannover, Germany. birthe.tegtmeyer@twincore.de.ORCID 0000-0003-3875-0707
Jolanta KolodziejekInstitute of Virology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. jolanta.kolodziejek@vetmeduni.ac.at.
Norbert NowotnyInstitute of Virology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. norbert.nowotny@vetmeduni.ac.at.ORCID 0000-0002-3548-571X
Eike SteinmannDepartment of Molecular and Medical Virology, Ruhr-University Bochum, 44801 Bochum, Germany. eike.steinmann@rub.de.
Jessika-M V CavalleriUniversity Equine Clinic - Internal Medicine, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. jessika.cavalleri@vetmeduni.ac.at.ORCID 0000-0002-8575-7402
University of Veterinary Medicine Vienna · ATHelmholtz Centre for Infection Research · DERuhr University Bochum · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prevalence studies have demonstrated a global distribution of equine hepacivirus (EqHV), a member of the family Flaviviridae. However, apart from a single case of vertical transmission, natural routes of EqHV transmission remain elusive. Many known flaviviruses are horizontally transmitted between hematophagous arthropods and vertebrate hosts. This study represents the first investigation of potential EqHV transmission by mosquitoes. More than 5000 mosquitoes were collected across Austria and analyzed for EqHV ribonucleic acid (RNA) by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Concurrently, 386 serum samples from horses in eastern Austria were analyzed for EqHV-specific antibodies by luciferase immunoprecipitation system (LIPS) and for EqHV RNA by RT-qPCR. Additionally, liver-specific biochemistry parameters were compared between EqHV RNA-positive horses and EqHV RNA-negative horses. Phylogenetic analysis was conducted in comparison to previously published sequences from various origins. No EqHV RNA was detected in mosquito pools. Serum samples yielded an EqHV antibody prevalence of 45.9% (177/386) and RNA prevalence of 4.15% (16/386). EqHV RNA-positive horses had significantly higher glutamate dehydrogenase (GLDH) levels (

Indexed as

AnimalsAntibodies, ViralAustriaCulicidaeFemaleGlutamate DehydrogenaseHepacivirusHepatitis CHorse DiseasesHorsesLiverMalePhylogenyPrevalenceRNA, ViralAntibodies, ViralGlutamate DehydrogenaseRNA, Viralarbovirusflavivirushematophagous arthropodhepacivirus Ahepatitisinsectsmosquito-borne virusvirus transmission

Identifiers

PMID31683893
PMCPMC6893842
OpenAlexW2983380455

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.