ArticleResearch in veterinary science2019
Characterisation of Crandell-Rees Feline Kidney (CRFK) cells as mesenchymal in phenotype.
Article in Research in veterinary science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Protective Effects Assessment of Combined Extracts fromVeterinary sciences · 2026Article
- Whole-genome sequencing of CRFK and PG-4 cells to infer the phenotype of the original donor cats.Companion animal health and genetics · 2026Article
- Nephroprotective effect of spexin in dogs and cats.BMC veterinary research · 2026Article
- Unraveling TGF-β1's Role in Mediating Fibrosis and Cell Death in Feline Kidney Cells.Animals : an open access journal from MDPI · 2025Article
- Detection ofPathogens (Basel, Switzerland) · 2024Article
- Feline coronavirus influences the biogenesis and composition of extracellular vesicles derived from CRFK cells.Frontiers in veterinary science · 2024Article
- Cross-reactivity of anti-human programmed cell death ligand 1 (PD-L1) monoclonal antibody, clone 28-8 against feline PD-L1.The Journal of veterinary medical science · 2023Article
- Crude Extracts ofViruses · 2023Article
- Choosing a cellular model to study SARS-CoV-2.Frontiers in cellular and infection microbiology · 2022Review
- Protective Effect of Natural Antioxidant Compounds on Methimazole Induced Oxidative Stress in a Feline Kidney Epithelial Cell Line (CRFK).Veterinary sciences · 2021Article
- Feline adipose-derived mesenchymal stem cells induce effector phenotype and enhance cytolytic function of CD8+ T cells.Stem cell research & therapy · 2021Article
- Inhibition of Crandell-Rees Feline Kidney cell proliferation by X-ray-induced senescence.The Journal of veterinary medical science · 2021Article
- Prevalence of autoantibodies that bind to kidney tissues in cats and association risk with antibodies to feline viral rhinotracheitis, calicivirus, and panleukopenia.Journal of veterinary science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Crandell-Rees Feline Kidney Cell (CRFK) is an immortalised cell line derived from the feline kidney that is utilised for the growth of certain vaccinal viruses. Confusion exists as to whether CRFK are epithelial or mesenchymal in phenotype. The aim of this study was to characterise CRFK cells via immunofluorescence, enzyme cytochemistry, western blotting, RT-qPCR for S100A4 and comparison to primary feline proximal tubular epithelial cells (FPTEC) and feline cortical fibroblasts (FCF). CRFK cells were of fusiform morphology and appeared similar to FCF. CRFK expressed the mesenchymal intermediate filament (IF) protein vimentin together with two cell adhesion molecules associated with feline fibroblasts (CD29 and CD44), and lacked expression of the epithelial IF cytokeratin, myogenic IF desmin and endothelial marker von Willebrand factor (vWF). In addition, CRFK did not demonstrate brush border enzyme activity typical of FPTEC. S100A4 gene expression, implicated in both neoplastic transformation and epithelial to mesenchymal transition, was highly upregulated in CRFK in comparison to the primary feline renal cells. CRFK appear phenotypically similar to fibroblasts, rather than tubular epithelial cells, and may have undergone neoplastic transformation or epithelial-to-mesenchymal transition after extensive passaging. This finding may have potential implications for future research utilising this cell line.
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