Evidence map›Paper›PMID 31681738›Full record

ReviewFrontiers in bioengineering and biotechnology2019

Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems.

Khushal Khambhati, Gargi Bhattacharjee, Nisarg Gohil, Darren Braddick, Vishwesh Kulkarni, Vijai Singh

Open access · goldAbstract readReview
In one paragraph

Review in Frontiers in bioengineering and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed, 1 pooled it
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 1 synthesis or guideline pooled it, 126 citations in OpenAlex.

  1. Pooled it
  2. Artificial cells with liquid-liquid phase separation-regulated cell-free protein synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Review
  4. Article
  5. Developing anACS synthetic biology · 2025
    Article
  6. Substrate recognition by a peptide-aminoacyl-tRNA ligase.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection.bioRxiv : the preprint server for biology · 2025
    Article
  8. Article
  9. BeyondChemical reviews · 2025
    Review
  10. Article
  11. Review
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  13. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Khushal KhambhatiDepartment of Biological Sciences and Biotechnology, Institute of Advanced Research, Gandhinagar, India.
Gargi BhattacharjeeDepartment of Biological Sciences and Biotechnology, Institute of Advanced Research, Gandhinagar, India.
Nisarg GohilDepartment of Biological Sciences and Biotechnology, Institute of Advanced Research, Gandhinagar, India.
Darren BraddickDepartment of R&D, Cementic S.A.S., Genopole, Paris, France.
Vishwesh KulkarniSchool of Engineering, University of Warwick, Coventry, United Kingdom.
Vijai SinghDepartment of Biological Sciences and Biotechnology, Institute of Advanced Research, Gandhinagar, India.
Indian Institute of Advanced Research · INGenopole (France) · FRUniversity of Warwick · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and machineries required for the synthesis of the desired proteins. It has the potential to overcome loopholes in the current

Indexed as

biocontainmentCFPShigh-throughput proteinssynthetic biologytherapeuticsvirus-like particles

Identifiers

PMID31681738
PMCPMC6797904
OpenAlexW2979837817

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.