ArticleJournal of extracellular vesicles2019
Determination of variability due to biological and technical variation in urinary extracellular vesicles as a crucial step in biomarker discovery studies.
Article in Journal of extracellular vesicles, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles.Journal of extracellular vesicles · 2021Guideline
- Urinary Extracellular Vesicles Biomarkers in CKD: Clinical Laboratory Translation.Diagnostics (Basel, Switzerland) · 2026Review
- Methodological and Short-Term Diurnal Variation in Surface and Cargo Proteins in Plasma Extracellular Vesicles.Current issues in molecular biology · 2026Article
- Multiomics Profiling of Extracellular Vesicles Supports Their Involvement in Endothelial Senescence-Associated Vascular Dysfunction.Journal of extracellular biology · 2025Article
- Defining Biological Variability, Analytical Precision and Quantitative Biophysiochemical Characterization of Human Urinary Extracellular Vesicles.Journal of extracellular vesicles · 2025Article
- Prostate cancer reshapes the secreted and extracellular vesicle urinary proteomes.Nature communications · 2024Article
- Exploring the role of urinary extracellular vesicles in kidney physiology, aging, and disease progression.American journal of physiology. Cell physiology · 2023Review
- Protein Biomarker Discovery Studies on Urinary sEV Fractions Separated with UF-SEC for the First Diagnosis and Detection of Recurrence in Bladder Cancer Patients.Biomolecules · 2023Article
- A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis.Journal of extracellular vesicles · 2022Article
- Single Extracellular Vesicle Analysis Performed by Imaging Flow Cytometry and Nanoparticle Tracking Analysis Evaluate the Accuracy of Urinary Extracellular Vesicle Preparation Techniques Differently.International journal of molecular sciences · 2021Article
- Urinary extracellular vesicles: Assessment of pre-analytical variables and development of a quality control with focus on transcriptomic biomarker research.Journal of extracellular vesicles · 2021Article
- Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals.Scientific reports · 2021Article
- Technological Approaches in the Analysis of Extracellular Vesicle Nucleotide Sequences.Frontiers in bioengineering and biotechnology · 2021Review
- Label-Free Liquid Chromatography-Mass Spectrometry Proteomic Analysis of Urinary Identification in Diabetic Vascular Dementia in a Han Chinese Population.Frontiers in aging neuroscience · 2021Article
- Urinary Extracellular Vesicles Magic Particles for Biomarker Discovery.Advances in experimental medicine and biology · 2021Article
- Glass Fiber-Supported Hybrid Monolithic Spin Tip for Enrichment of Phosphopeptides from Urinary Extracellular Vesicles.Analytical chemistry · 2020Article
- Switching Homes: How Cancer Moves to Bone.International journal of molecular sciences · 2020Review
- Urinary Biomarkers in Bladder Cancer: Where Do We Stand and Potential Role of Extracellular Vesicles.Cancers · 2020Review
- MicroRNAs in Small Extracellular Vesicles Indicate Successful Embryo Implantation during Early Pregnancy.Cells · 2020Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urinary extracellular vesicles (EVs) are an attractive source of biomarkers for urological diseases. A crucial step in biomarker discovery studies is the determination of the variation parameters to perform a sample size calculation. In this way, a biomarker discovery study with sufficient statistical power can be performed to obtain biologically significant biomarkers. Here, a variation study was performed on both the protein and lipid content of urinary EVs of healthy individuals, aged between 52 and 69 years. Ultrafiltration (UF) in combination with size exclusion chromatography (SEC) was used to isolate the EVs from urine. Different experimental variation set-ups were used in this variation study. The calculated standard deviations (SDs) of the 90% least variable peptides and lipids did not exceed 2 and 1.2, respectively. These parameters can be used in a sample size calculation for a well-designed biomarker discovery study at the cargo of EVs.
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