ArticleJournal of animal physiology and animal nutrition2020
A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets.
Article in Journal of animal physiology and animal nutrition, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Impact of Regular Intake of Microalgae on Nutrient Supply and Cardiovascular Risk Factors: Results from the NovAL Intervention Study.Nutrients · 2023Trial
- From Pond to Plate: Tailored Functional Proteins for Next-Gen Microalgal Food.Foods (Basel, Switzerland) · 2026Review
- Transmission electron microscopy characterisation of Spirulina bioplastics.Journal of microscopy · 2026Article
- Microalgal Bioethanol Production for Sustainable Development: Current Status and Future Prospects.Indian journal of microbiology · 2025Article
- Microalgal diversity, nutritional profiles, and applications in poultry nutrition: A comprehensive review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Review
- Unlocking the feed supplement potentials of blue-green alga (spirulina) in broiler nutrition: a comprehensive review.Tropical animal health and production · 2025Review
- The combined effects of extended feeding with a high level of Arthrospira platensis and a commercial enzyme mix or porcine pancreatin on broilers' blood cells, plasma metabolites and liver lipid profile.BMC veterinary research · 2025Article
- Review
- Improving protein hydrolysis and digestibility inHeliyon · 2025Article
- Efficient Green Extraction of Nutraceutical Compounds fromFoods (Basel, Switzerland) · 2024Article
- Chemical Composition, Bioactivities, and Applications of Spirulina (Foods (Basel, Switzerland) · 2024Review
- Optimised phycoerythrin extraction method fromHeliyon · 2024Article
- Effects of high dietary inclusion of Arthrospira platensis, either extruded or supplemented with a super-dosing multi-enzyme mixture, on broiler growth performance and major meat quality parameters.BMC veterinary research · 2024Article
- Microalgae as an Alternative Mineral Source in Poultry Nutrition.Veterinary sciences · 2024Review
- Impact of high Spirulina diet, extruded or supplemented with enzymes, on blood cells, systemic metabolites, and hepatic lipid and mineral profiles of broiler chickens.Frontiers in veterinary science · 2024Article
- Impact of long-term feeding a high level of Spirulina combined with enzymes on growth performance, carcass traits and meat quality in broiler chickens.Frontiers in veterinary science · 2024Article
- Microalgae as a Sustainable Source of Antioxidants in Animal Nutrition, Health and Livestock Development.Antioxidants (Basel, Switzerland) · 2023Review
- Digestive Constraints ofFoods (Basel, Switzerland) · 2022Review
- Effect on Broiler Production Performance and Meat Quality of FeedingAnimals : an open access journal from MDPI · 2022Article
- Recalcitrant cell wall ofAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The main goal of this study was to test a rational combination of pre-selected carbohydrate-active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two-enzyme constituted mixture (Mix), composed by a lysozyme and a α-amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7-fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34-fold increase in protein content (p = .018) relative to the control. Similarly, n-6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n-6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two-enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost-effective advantage use of microalgae in animal feed industry.
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Registered trials
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