ArticleJournal of experimental botany2020
Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 85 citations in OpenAlex.
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- Evolution and functional characterization of Populus salt stress-responsive calcineurin B-like protein-interacting protein kinases.Plant cell reports · 2024Article
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- Genetic diversity and population structure analysis of 418 tomato cultivars based on single nucleotide polymorphism markers.Frontiers in plant science · 2024Article
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- Hydrogen sulfide improves salt tolerance through persulfidation of PMA1 in Arabidopsis.Plant cell reports · 2023Article
- Control of ovule development inHorticulture research · 2023Article
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- Interaction Network Construction and Functional Analysis of the Plasma Membrane HInternational journal of molecular sciences · 2023Article
- Growth-regulating factor 15-mediated gene regulatory network enhances salt tolerance in poplar.Plant physiology · 2023Article
- Advances in the Research on Plant WRKY Transcription Factors Responsive to External Stresses.Current issues in molecular biology · 2023Review
- Article
- How do plants maintain pH and ion homeostasis under saline-alkali stress?Frontiers in plant science · 2023Review
- CC-type glutaredoxin, MeGRXC3, associates with catalases and negatively regulates drought tolerance in cassava (Manihot esculenta Crantz).Plant biotechnology journal · 2022Article
- Role of PsnWRKY70 in Regulatory Network Response to Infection withInternational journal of molecular sciences · 2022Article
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Authors and funding
18 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis.
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