Evidence map›Paper›PMID 31679136›Full record

ArticleInternational urology and nephrology2020

Urinary micro-RNA expressions and protein concentrations may differentiate bladder cancer patients from healthy controls.

Gökçe Güllü Amuran, Ilker Tinay, Deniz Filinte, Can Ilgin, Irem Peker Eyüboğlu, Mustafa Akkiprik

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In one paragraph

Article in International urology and nephrology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 3 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 3 syntheses or guidelines pooled it, 32 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Review
  5. Review
  6. Article
  7. Review
  8. An Overview of Angiogenesis in Bladder Cancer.Current oncology reports · 2023
    Review
  9. Review
  10. Review
  11. The Role of Novel Bladder Cancer Diagnostic and Surveillance Biomarkers-What Should a Urologist Really Know?International journal of environmental research and public health · 2022
    Review
  12. Circulating exosomal miRNAs and cancer early diagnosis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2022
    Review
  13. Review
  14. Review
  15. [miR-let-7c-5p inhibits invasion and migration of bladder cancer cells by targeting HMGA2].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021
    Article
  16. Review
  17. What can urinary exosomes tell us?Cellular and molecular life sciences : CMLS · 2021
    Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Gökçe Güllü AmuranDepartment of Medical Biology, School of Medicine, Marmara University, Başıbüyük Campus, Başıbüyük Mah. Maltepe Başıbüyük Yolu Sok. No:9/2, Maltepe, 34854, Istanbul, Turkey.
Ilker TinayDepartment of Urology, School of Medicine, Marmara University, 34854, Istanbul, Turkey.
Deniz FilinteDepartment of Pathology, School of Medicine, Marmara University, 34854, Istanbul, Turkey.
Can IlginDepartment of Public Health, School of Medicine, Marmara University, 34899, Istanbul, Turkey.
Irem Peker EyüboğluDepartment of Medical Biology, School of Medicine, Marmara University, Başıbüyük Campus, Başıbüyük Mah. Maltepe Başıbüyük Yolu Sok. No:9/2, Maltepe, 34854, Istanbul, Turkey.
Mustafa AkkiprikDepartment of Medical Biology, School of Medicine, Marmara University, Başıbüyük Campus, Başıbüyük Mah. Maltepe Başıbüyük Yolu Sok. No:9/2, Maltepe, 34854, Istanbul, Turkey. makkiprik@marmara.edu.tr.
Marmara University · TRMaltepe University · TR

Funding

Marmara Üniversitesi BAPKO-SAG-C-DRP-1310160441
6 · The paper itself

Abstract

purposeTo determine expression differences of urine exosomal miR-19b1-5p, 21-5p, 136-3p, 139-5p, 210-3p and concentration differences of urinary BLCA-4, NMP22, APE1/Ref1, CRK, VIM between bladder cancer, follow-up patients, and control samples, to evaluate diagnostic importance of these differences and establish a diagnostic panel for bladder cancer.

methodsUrine samples of 59 bladder cancer patients, 34 healthy controls, and 12 follow-up patients without recurrence were enrolled to this study. Real-time PCR and ELISA were performed to determine urine exosomal miR-19b1-5p, 21-5p, 136-3p, 139-5p, 210-3p expressions and urinary BLCA-4, NMP22, APE1/Ref1, CRK, VIM, creatinine concentrations. Logistic regression analyses were performed to determine the diagnostic panel, the sensitivity, and specificity of the panel assessed by the ROC curve analysis. p values < 0.05 were considered statistically significant.

resultsIn bladder cancer risk groups, mir-139, -136, -19 and 210 expressions or positivity were found to be different and concentrations of urinary Ape1/Ref1, BLCA4, CRK, and VIM increased by twofold on average compared to healthy controls. Logistic regression and ROC analyses revealed that panel could differentiate bladder cancer patients from healthy controls with 80% sensitivity and 88% specificity (AUC = 0.899), low-risk patients from controls with 93% sensitivity and 95.5% specificity (AUC = 0.976). Despite the low number of samples, our findings suggest that urine exosomal miR-19b1-5p, 136-3p, 139-5p expression, and urinary APE1/Ref1, BLCA-4, CRK concentrations are promising candidates in terms of bladder cancer diagnosis.

conclusionsAlthough our panel has great sensitivity for early detection of BC, it needs to be validated in larger populations.

Indexed as

Urinary Bladder NeoplasmsBiomarkers, TumorDiagnosis, DifferentialExosomesFemaleGene Expression Regulation, NeoplasticHumansMaleMicroRNAsMiddle AgedNeoplasm ProteinsNuclear ProteinsROC CurveUrinalysisBiomarkers, TumorBLCA-4 protein, humanMicroRNAsMIRN139 microRNA, humanNeoplasm ProteinsNuclear ProteinsBladder cancerBladder cancer diagnosisUrinary biomarkerUrinary proteinUrine exosomal miRNA

Identifiers

PMID31679136
OpenAlexW2989225794

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.