Evidence map›Paper›PMID 31678132›Full record

ArticleJournal of microbiological methods2019

Quantitative assessment of bacterial growth phase utilizing flow cytometry.

K M Khomtchouk, M Weglarz, L A Bekale, I Koliesnik, P L Bollyky, P L Santa Maria

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiological methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

K M KhomtchoukDepartment of Otolaryngology, Head and Neck Surgery, Stanford University, USA.
M WeglarzStanford Shared FACS Facility, Stanford University, USA.
L A BekaleDepartment of Otolaryngology, Head and Neck Surgery, Stanford University, USA.
I KoliesnikDepartment of Medicine, Infectious Diseases, Stanford University, USA.
P L BollykyDepartment of Medicine, Infectious Diseases, Stanford University, USA.
P L Santa MariaDepartment of Otolaryngology, Head and Neck Surgery, Stanford University, USA. Electronic address: petersantamaria@stanford.edu.
Stanford University · USAmerican College of Surgeons · US

Funding

Lymph Node Extracellular Matrix in Antigen Presentation and Immune RegulationR01DK096087 · NIDDK · STANFORD UNIVERSITY · PI BOLLYKY, PAUL L · 2012 to 2021
$3.4M
Pf Bacteriophages in the Pathophysiology of Cystic Fibrosis and Pseudomonas aeruginosa InfectionR21AI137432 · NIAID · STANFORD UNIVERSITY · PI BOLLYKY, PAUL L · 2018 to 2019
$437k
Biofilms and Bacteriophages in Chronic Wound InfectionsR21AI133370 · NIAID · STANFORD UNIVERSITY · PI BOLLYKY, PAUL L · 2017 to 2017
$319k
BD Canto II RUO CytometerS10RR027431 · NCRR · STANFORD UNIVERSITY · PI NOLAN, GARRY P · 2010 to 2010
$236k
NCATS NIH HHS L40 TR002532NCRR NIH HHS S10 RR027431NIAID NIH HHS R21 AI133370NIAID NIH HHS R21 AI137432NIDDK NIH HHS R01 DK096087
6 · The paper itself

Abstract

Flow cytometry is currently underutilized for bacterial phenotyping and standard microbiological techniques do not provide phenotypic information about the state of the bacterial disease. Pseudomonas aeruginosa is a human pathogen of increased importance in public health due to both the ability to cause chronic diseases and the prevalence of functionally different subsets that can be difficult to treat and diagnose. In the present study, we used flow cytometry to analyze the growth phase of P. aeruginosa. A simple method for single cell quantitative detection of bacterial biofilm and planktonic cells was established with a combination of membrane permeable (SYTO 60) and impermeable (TOTO-1) dyes plus the addition of polystyrene counting beads. The specificity of the dye combination for biofilm detection was determined by comparison with impaired biofilm forming strains of P. aeruginosa LasI/RhlI-/- and ∆PfPhage. Results suggest that flow cytometric bacterial phenotyping serves as an expandable platform that may be useful for enumeration of population level variation in P. aeruginosa studies.

Indexed as

BiofilmsColoring AgentsFlow CytometryPhenotypePseudomonas aeruginosaSingle-Cell AnalysisStaining and LabelingThiazolesColoring AgentsThiazolesTOTO-1 thiazole orange dye

Identifiers

PMID31678132
PMCPMC6957528
OpenAlexW2986080279

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.