ArticleeLife2019
An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms.
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In one paragraph
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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105citing papers in PubMed
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Who cites it
105 citing papers in PubMed.
- Optimizing microhomology-based genome editing by engineering DNA polymerase θ for improved efficiency and reduced on-target indels.Cell reports methods · 2026Article
- Conserved Transcriptomic Signatures of Sirt6 Activity: A Cross-Species RNA-seq Meta-analysis.bioRxiv : the preprint server for biology · 2026Article
- Highly frequent undesired insertional mutagenesis during Drosophila genome editing.PLoS genetics · 2026Article
- Article
- Mmp2 regulates basement membrane remodeling and dedifferentiation of the visceral musculature during Drosophila metamorphosis.Scientific reports · 2026Article
- Two lysosomal genes ATP13A2 and GBA1 interact to drive neurodegeneration.Molecular neurodegeneration · 2026Article
- Multimodal social context modulates larval behavior inScience advances · 2026Article
- RNAi-based screen for pigmentation iniScience · 2026Article
- CrisprBuildr: an open-source application for CRISPR-mediated genome engineering in Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- Article
- dFlpTag, a RMCE-based tool for simultaneous endogenous protein tagging and cell labeling in Drosophila.Scientific reports · 2025Article
- Nascent dendrite branches initiated by a localized burst of Spire-dependent actin polymerization.Development (Cambridge, England) · 2025Article
- Mushroom bodies tiny regulates Sidekick localization to tricellular adherens junctions.Development (Cambridge, England) · 2025Article
- RNAi-based screen for pigmentation inbioRxiv : the preprint server for biology · 2025Article
- Structure, function, and quantitative biology of the Drosophila gut microbiome.Current opinion in microbiology · 2025Review
- Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells.Nature communications · 2025Article
- Repulsive interactions instruct synaptic partner matching in an olfactory circuit.bioRxiv : the preprint server for biology · 2025Article
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- A library of lineage-specific driver lines connects developing neuronal circuits to behavior in theeLife · 2025Article
- Functional analysis of pathogenic variants in LAMB1-related leukoencephalopathy reveals genotype-phenotype correlations and suggests its role in glial cells.Human molecular genetics · 2025Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors.
Oguz KancaDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.ORCID 0000-0001-5438-0879
Jonathan ZirinHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.
Jorge Garcia-MarquesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
Shannon Marie KnightHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.
Donghui Yang-ZhouHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.
Gabriel AmadorHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.
Hyunglok ChungDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Zhongyuan ZuoDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Liwen MaDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Yuchun HeDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Wen-Wen LinDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Ying FangDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Ming GeDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Shinya YamamotoDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Karen L SchulzeDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.ORCID 0000-0002-1368-729X
Yanhui HuHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.
Allan C SpradlingDepartment of Embryology, Howard Hughes Medical Institute, Carnegie Institution for Science, Baltimore, United States.ORCID 0000-0002-5251-1801
Stephanie E MohrHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.ORCID 0000-0001-9639-7708
Norbert PerrimonHoward Hughes Medical Institute, Harvard Medical School, Boston, United States.ORCID 0000-0001-7542-472X
Hugo J BellenDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.ORCID 0000-0001-5992-5989
Funding
Transposon induced disruption of most Drosophila genesR01GM067858 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI BELLEN, HUGO J · 2003 to 2020
$15.7MNew collections of dsRNAs and overexpression miRNA constructs to supplement RNAiR01GM067761 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI PERRIMON, NORBERT · 2003 to 2019
$15.5MDrosophila Transgenic RNAi Resource ProjectR01GM084947 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2008 to 2019
$9.8MSteps towards a paternal gene activation therapy for Angelman syndromeU54HD083092 · NICHD · BAYLOR COLLEGE OF MEDICINE · PI LEE, BRENDAN, NELSON, DAVID LOREN · 2014 to 2019
$7.8MFunctional genomics resources for the Drosophila - TR&D3P41GM132087 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2019 to 2023
$6.8MA Comprehensive Resource for Manipulating the Drosophila GenomeR24OD031447 · OD · BAYLOR COLLEGE OF MEDICINE · PI HUGO J BELLEN, Oguz Kanca · 2021 to 2026
$5.2MNext-generation Drosophila cell lines to elucidate the cellular basis of human diseasesR24OD019847 · OD · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT, SIMCOX, AMANDA · 2017 to 2020
$2.7MNICHD NIH HHS U54 HD083092NIGMS NIH HHS P41 GM132087NIGMS NIH HHS R01 GM067761NIGMS NIH HHS R01 GM067858NIGMS NIH HHS R01GM067858NIGMS NIH HHS R01 GM084947NIH HHS R24 OD019847NIH HHS R24 OD031447
Abstract
We previously reported a CRISPR-mediated knock-in strategy into introns of
Indexed as
Clustered Regularly Interspaced Short Palindromic RepeatsHomologous RecombinationAnimalsDNADNA, Single-StrandedDrosophilaGene Knock-In TechniquesMutagenesis, InsertionalDNADNA, Single-StrandedCRIMICCRISPRD. melanogasterdrop-indrosophilageneticsgenomicshomologous recombination
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Read underepoch 390
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.