ArticleChembiochem : a European journal of chemical biology2020
Using a PCR-Based Method To Analyze and Model Large, Heterogeneous Populations of DNA.
Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Immobilization-free SELEX for aptamer discovery targeting colorectal cancer-derived small extracellular vesicles.Journal of nanobiotechnology · 2025Article
- Strategies for developing DNA-encoded libraries beyond binding assays.Nature chemistry · 2022Review
- Quantitation of DNA-Encoded Libraries by qPCR.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Advancing DNA Steganography with Incorporation of Randomness.Chembiochem : a European journal of chemical biology · 2020Article
- Using a PCR-Based Method To Analyze and Model Large, Heterogeneous Populations of DNA.Chembiochem : a European journal of chemical biology · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
The study of populations of large size and high diversity is limited by the capability of collecting data. Moreover, for a pool of individuals, each associated with a unique characteristic feature, as the pool size grows, the possible interactions increase exponentially and quickly go beyond the limit of computation and experimental studies. Herein, the design of DNA libraries with various diversity is reported. By using a facile analytical method based on real-time PCR, the diversity of a pool of DNA can be evaluated to allow extraordinarily high heterogenicity (e.g., >1 trillion). It is demonstrated that these DNA libraries can be used to model heterogeneous populations; these libraries exhibit functions such as self-protection, suitability for biased expansion, and the possibility to evolve into amorphous structures. The method has shown the remarkable power of parallel computing with DNA, since it can resemble an analogue computer and be applied in selection-based biotechnology methods, such as DNA-encoded chemical libraries. As a chemical approach to solve problems traditionally for genetic and statistical analysis, the method provides a quick and cost-efficient evaluation of library diversity for intermediate steps through a selection process.
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Registered trials
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