ArticleJournal of biomedical optics2019
Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector.
Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.International journal of molecular sciences · 2023Review
- Axial scanning of dual focus to improve light sheet microscopy.Biomedical optics express · 2022Article
- 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy.Communications biology · 2022Article
- Exploring the human cerebral cortex using confocal microscopy.Progress in biophysics and molecular biology · 2022Article
- Identification of microplastics using 4-dimethylamino-4'-nitrostilbene solvatochromic fluorescence.Microscopy research and technique · 2021Article
- Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques.Frontiers in neuroanatomy · 2021Article
- Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy.Biomedical optics express · 2020Article
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) has been established as a gold standard method to improve image quality. The selective line detection of a complementary metal–oxide–semiconductor camera (CMOS) working in rolling shutter mode allows the rejection of out-of-focus and scattered light, thus reducing background signal during image formation. Most modern CMOS have two rolling shutters, but usually only a single illuminating beam is used, halving the maximum obtainable frame rate. We report on the capability to recover the full image acquisition rate via dual confocal DSLM by using an acousto-optic deflector. Such a simple solution enables us to independently generate, control and synchronize two beams with the two rolling slits on the camera. We show that the doubling of the imaging speed does not affect the confocal detection high contrast.
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Registered trials
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