ArticleTransgenic research2020
Overexpression of the maize γ-tocopherol methyltransferase gene (ZmTMT) increases α-tocopherol content in transgenic Arabidopsis and maize seeds.
Article in Transgenic research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Comparative metabolomic analysis of leaves and kernels in wild type andFood chemistry. Molecular sciences · 2026Article
- Natural Ageing-Related Alterations of Biological Markers in Maize Seeds Under Ex-Situ Conservation.International journal of molecular sciences · 2025Article
- Sequence Variation and In Silico Protein Characterization ofInternational journal of molecular sciences · 2025Article
- An alternative splicing caused by a natural variation in BnaC02.VTE4 gene affects vitamin E and glucosinolate content in rapeseed (Brassica napus L.).Plant biotechnology journal · 2025Article
- Vitamin E in Plants: Biosynthesis Pathways, Biofortification Strategies, and Regulatory Dynamics.International journal of molecular sciences · 2025Review
- Calcium and Magnesium Regulation of Kernel Sugar Content in Maize: Role of Endogenous Hormones and Antioxidant Enzymes.International journal of molecular sciences · 2024Article
- Heterologous Expression of SunflowerPlants (Basel, Switzerland) · 2024Article
- A Summary of Two Decades of QTL and Candidate Genes That Control Seed Tocopherol Contents in Maize (Genes · 2024Review
- ROS scavenging and ion homeostasis is required for the adaptation of halophyteFrontiers in plant science · 2022Article
- Assessment of Biofortification Approaches Used to Improve Micronutrient-Dense Plants That Are a Sustainable Solution to Combat Hidden Hunger.Journal of soil science and plant nutrition · 2022Review
- Antioxidant and Signaling Role of Plastid-Derived Isoprenoid Quinones and Chromanols.International journal of molecular sciences · 2021Review
- Vitamins in Cereals: A Critical Review of Content, Health Effects, Processing Losses, Bioaccessibility, Fortification, and Biofortification Strategies for Their Improvement.Frontiers in nutrition · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The vitamin E family includes tocopherols and tocotrienols, which are essential lipid-soluble antioxidants necessary for human and livestock health. The seeds of many plant species, including maize, have high gamma (γ)-tocopherol but low alpha (α)-tocopherol contents; however, α-tocopherol is the most effective antioxidant. Therefore, it is necessary to optimize the tocopherol composition in plants. α-Tocopherol is synthesized from γ-tocopherol by γ-tocopherol methyltransferase (γ-TMT, VTE4) in the final step of the tocopherol biosynthetic pathway. In the present study, the full-length coding sequence (CDS) of γ-TMT was isolated from Zea mays, named ZmTMT. The ZmTMT CDS was 1059 bp in size, encoding 352 amino acids. Recombinant ZmTMT was expressed in Escherichia coli and the purified protein effectively converted γ-tocopherol into α-tocopherol in vitro. A comparison of enzyme activities showed that the activity of ZmTMT was higher than that of GmTMT2a (Glycine max) and AtTMT (Arabidopsis thaliana). Overexpression of ZmTMT increased the α-tocopherol content 4-5-fold in transgenic Arabidopsis and around 6.5-fold in transgenic maize kernels, and increased the α-/γ-tocopherol ratio to approximately 15 and 17, respectively. These results show that it is feasible to overexpress ZmTMT to optimize the tocopherol composition in maize; such a corn product might be useful in the feed industry in the near future.
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