ArticleBiomicrofluidics2019
Electrophoretic exclusion microscale sample preparation for cryo-EM structural determination of proteins.
Article in Biomicrofluidics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Dielectrophoresis reveals stimulus-induced remodeling of insulin granule subpopulations.Biophysical journal · 2026Article
- Dielectrophoresis Reveals Stimulus-Induced Remodeling of Insulin Granule Subpopulations.bioRxiv : the preprint server for biology · 2025Article
- Article
- Filtering and continuously separating microplastics from water using electric field gradients formed electrochemically in the absence of buffer.Chemical science · 2021Article
- Dielectrophoresis of proteins: experimental data and evolving theory.Analytical and bioanalytical chemistry · 2020Article
- Festschrift for Professor Hsueh-Chia Chang.Biomicrofluidics · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Transmission electron microscopy (TEM) of biological samples has a long history and has provided many important insights into fundamental processes and diseases. While great strides have been made in EM data collection and data processing, sample preparation is still performed using decades-old techniques. Those sample preparation methods rely on extensive macroscale purification and concentration to achieve homogeneity suitable for high-resolution analyses. Noting that relatively few bioparticles are needed to generate high-quality protein structures, this work uses microfluidics that can accurately and precisely manipulate and deliver bioparticles to grids for imaging. The use of microfluidics enables isolation, purification, and concentration of specific target proteins at these small scales and does so in a relatively short period of time (minutes). These capabilities enable imaging of more dilute solutions and obtaining pure protein images from mixtures. In this system, spatially isolated, purified, and concentrated proteins are transferred directly onto electron microscopy grids for imaging. The processing enables imaging of more dilute solutions, as low as 5 × 10
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.