ArticleScientific reports2019
Identification and evolution of glucosinolate sulfatases in a specialist flea beetle.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Field investigation of glucosinolates and morphological traits in mitigating Psylliodes chrysocephala larval infestation through crop varieties and companion planting.Pest management science · 2026Article
- Host Rejection of the White Mustard (Sinapis alba) by a Major Oilseed Rape Pest Is Driven by the Avoidance of Sinalbin.Journal of chemical ecology · 2026Article
- Cooked Broccoli Alters Cecal Microbiota and Impacts Microbial Metabolism of Glucoraphanin in Lean and Obese Mice.Molecular nutrition & food research · 2025Article
- Phytochemical drivers of insect herbivory: a functional toolbox to support agroecological diversification.Royal Society open science · 2024Article
- New Insights into theJournal of agricultural and food chemistry · 2023Review
- Unique metabolism of different glucosinolates in larvae and adults of a leaf beetle specialised on Brassicaceae.Scientific reports · 2022Article
- Review
- Metabolization and sequestration of plant specialized metabolites in insect herbivores: Current and emerging approaches.Frontiers in physiology · 2022Review
- De Novo Whole-Genome Assembly of the Swede Midge (Contarinia nasturtii), a Specialist of Brassicaceae, Using Linked-Read Sequencing.Genome biology and evolution · 2021Article
- Article
- Identification of a Sulfatase that Detoxifies Glucosinolates in the Phloem-Feeding InsectFrontiers in plant science · 2021Article
- Hijacking the Mustard-Oil Bomb: How a Glucosinolate-Sequestering Flea Beetle Copes With Plant Myrosinases.Frontiers in plant science · 2021Article
- Glucosinolates: Natural Occurrence, Biosynthesis, Accessibility, Isolation, Structures, and Biological Activities.Molecules (Basel, Switzerland) · 2020Review
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucosinolates, a characteristic group of specialized metabolites found in Brassicales plants, are converted to toxic isothiocyanates upon herbivory. Several insect herbivores, including the cabbage stem flea beetle (Psylliodes chrysocephala), prevent glucosinolate activation by forming desulfo-glucosinolates. Here we investigated the molecular basis of glucosinolate desulfation in P. chrysocephala, an important pest of oilseed rape. Enzyme activity assays with crude beetle protein extracts revealed that glucosinolate sulfatase (GSS) activity is associated with the gut membrane and has narrow substrate specificity towards the benzenic glucosinolate sinalbin. In agreement with GSS activity localization in vivo, we identified six genes encoding arylsulfatase-like enzymes with a predicted C-terminal transmembrane domain, of which five showed GSS activity upon heterologous expression in insect cells. PcGSS1 and PcGSS2 used sinalbin and indol-3-ylmethyl glucosinolate as substrates, respectively, whereas PcGSS3, PcGSS4, and PcGSS5 showed weak activity in enzyme assays. RNAi-mediated knock-down of PcGSS1 and PcGSS2 expression in adult beetles confirmed their function in vivo. In a phylogenetic analysis of coleopteran and lepidopteran arylsulfatases, the P. chrysocephala GSSs formed a cluster within a coleopteran-specific sulfatase clade distant from the previously identified GSSs of the diamondback moth, Plutella xylostella, suggesting an independent evolution of GSS activity in ermine moths and flea beetles.
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