ArticleScientific reports2019
Identification of suitable controls for miRNA quantification in T-cells and whole blood cells in sepsis.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Whole blood miRNA expression signatures as predictors of neoadjuvant chemotherapy response in triple-negative breast cancer.Hereditas · 2026Article
- Circulating miR-122-5p, miR-125b-5p, and miR-27a-3p in Post-Mortem Whole Blood: An Exploratory Study of the Association with Sepsis-Related Death.Current issues in molecular biology · 2025Article
- Mitochondrial damage drives T-cell immunometabolic paralysis after major surgery.EMBO molecular medicine · 2025Article
- Reduced Expression of Selected Exosomal MicroRNAs Is Associated with Poor Outcomes in Patients with Acute Stroke Receiving Reperfusion Therapy-Preliminary Study.International journal of molecular sciences · 2025Article
- HeraNorm: an R shiny application for identifying optimal endogenous controls for miRNA and RNA assays in qPCR and ddPCR.Bioinformatics advances · 2025Article
- Article
- Association between diminished miRNA expression and the disease status of AML patients: Comparing to healthy control.Biomedical journal · 2023Article
- Role of miRNA dysregulation in sepsis.Molecular medicine (Cambridge, Mass.) · 2022Review
- miRNA Expression Profiling in Subcutaneous Adipose Tissue of Monozygotic Twins Discordant for HIV Infection: Validation of Differentially Expressed miRNA and Bioinformatic Analysis.International journal of molecular sciences · 2022Article
- MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytes.Journal of neuroinflammation · 2022Article
- Ketone Bodies Improve Human CD8Frontiers in medicine · 2022Article
- Cell-Crossing Functional Network Driven by microRNA-125a Regulates Endothelial Permeability and Monocyte Trafficking in Acute Inflammation.Frontiers in immunology · 2022Article
- Anti-inflammatory effects of miR-150 are associated with the downregulation of STAT1 in macrophages following lipopolysaccharide treatment.Experimental and therapeutic medicine · 2021Article
- Very-low-carbohydrate diet enhances human T-cell immunity through immunometabolic reprogramming.EMBO molecular medicine · 2021Article
- MiR-21 and Tocilizumab interactions improve COVID-19 myocarditis outcomes.Therapeutic advances in cardiovascular diseaseObservational
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complex immune dysregulation is a hallmark of sepsis. The occurring phases of immunosuppression and hyperinflammation require rapid detection and close monitoring. Reliable tools to monitor patient's immune status are yet missing. Currently, microRNAs are being discussed as promising new biomarkers in sepsis. However, no suitable internal control for normalization of miRNA expression by qPCR has been validated so far, thus hampering their potential benefit. We here present the first evaluation of endogenous controls for miRNA analysis in human sepsis. Novel candidate reference miRNAs were identified via miRNA microArray. TaqMan qPCR assays were performed to evaluate these microRNAs in T-cells and whole blood cells of sepsis patients and healthy controls in two independent cohorts. In T-cells, U48 and miR-320 proved suitable as endogenous controls, while in whole blood cells, U44 and miR-942 provided best stability values for normalization of miRNA quantification. Commonly used snRNA U6 exhibited worst stability in all sample groups. The identified internal controls have been prospectively validated in independent cohorts. The critical importance of housekeeping gene selection is emphasized by exemplary quantification of imuno-miR-150 in sepsis patients. Use of appropriate internal controls could facilitate research on miRNA-based biomarker-use and might even improve treatment strategies in the future.
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