Evidence map›Paper›PMID 31672639›Full record

ArticleJournal of the American College of Surgeons2020

Effect of Beta-Blockade on the Expression of Regulatory MicroRNA after Severe Trauma and Chronic Stress.

Camille G Apple, Elizabeth S Miller, Tyler J Loftus, Kolenkode B Kannan, Chase W Thompson, Maria Cecilia Lopez, Henry V Baker, Lyle L Moldawer, Philip A Efron, Alicia M Mohr

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American College of Surgeons, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Are Noradrenergic Transmission Reducing Drugs Antidepressants?Frontiers in behavioral neuroscience · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Camille G AppleDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Elizabeth S MillerDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Tyler J LoftusDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Kolenkode B KannanDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Chase W ThompsonDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Maria Cecilia LopezDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL.
Henry V BakerDepartment of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL.
Lyle L MoldawerDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Philip A EfronDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL.
Alicia M MohrDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida Health, Gainesville, FL. Electronic address: alicia.mohr@surgery.ufl.edu.
University of Florida Health · USUniversity of Florida · US

Funding

Molecular Biology in Burns and TraumaT32GM008721 · NIGMS · UNIVERSITY OF FLORIDA · PI Philip A Efron · 1999 to 2026
$5.0M
Chronic Stress and Anemia Recovery following Major TraumaR01GM105893 · NIGMS · UNIVERSITY OF FLORIDA · PI MOHR, ALICIA M · 2013 to 2021
$3.5M
NIGMS NIH HHS R01 GM105893NIGMS NIH HHS T32 GM008721
6 · The paper itself

Abstract

backgroundBeta-blockade administration after lung contusion, hemorrhagic shock, and chronic stress has been shown to improve bone marrow function, decrease hypercatecholaminemia, and reduce inflammation. MicroRNAs (miR) are critical biologic regulators that can downregulate gene expression by causing messenger RNA degradation or inhibition of translation. This study sought to expand our understanding of the molecular mechanisms underlying the reduced inflammatory response after the administration of beta-blockade (BB) in our rodent trauma model. STUDY

designMale Sprague-Dawley rats aged 8 to 9 weeks were randomized to lung contusion, hemorrhagic shock with daily restraint stress (LCHS/CS) or LCHS/CS plus propranolol (LCHS/CS+BB). Restraint stress occurred 2 hours daily after LCHS. Propranolol (10 mg/kg) was given daily until day 7. Total RNA and miR were isolated from bone marrow and genome-wide miR expression patterns were assayed. Bone marrow cytokine expression was determined with quantitative polymerase chain reaction.

resultsLCHS/CS led to significantly increased bone marrow expression of interleukin (IL) 1β, tumor necrosis factor-α, IL-6, nitric oxide, and plasma C-reactive protein. There were marked differences in expression of 45 miRs in the LCHS/CS+BB group compared with the LCHS/CS group when using a p value <0.001. Rno-miR-27a and miR-25 were upregulated 7- to 8-fold in the rodents who underwent LCHS/CS+BB compared with LCHS/CS alone, and this correlated with reduced bone marrow expression of IL-1β, tumor necrosis factor-α, IL-6, nitric oxide, and reduced plasma C-reactive protein in the LCHS/CS+BB group.

conclusionsThe genomic and miR expression patterns in bone marrow after LCHS/CS differed significantly compared with rodents that received propranolol after LCHS/CS. The use of BB after severe trauma can help mitigate persistent inflammation by upregulating Rno-miR-27a and miR-25 and reducing inflammatory cytokines in those who remain critically ill.

Indexed as

Stress, PhysiologicalAdrenergic beta-AntagonistsAnimalsChronic DiseaseContusionsInjury Severity ScoreLung InjuryMaleMicroRNAsPropranololRandom AllocationRatsRats, Sprague-DawleyRestraint, PhysicalShock, HemorrhagicAdrenergic beta-AntagonistsMicroRNAsPropranolol

Identifiers

PMID31672639
PMCPMC6934900
OpenAlexW2981869248

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.