Evidence map›Paper›PMID 31672442›Full record

ArticleMetabolism: clinical and experimental2019

Active pyruvate dehydrogenase and impaired gluconeogenesis in orthotopic hepatomas of rats.

Min Hee Lee, Ralph J DeBerardinis, Xiaodong Wen, Ian R Corbin, A Dean Sherry, Craig R Malloy, Eunsook S Jin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolism: clinical and experimental, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 1 country.

Min Hee LeeAdvanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Ralph J DeBerardinisChildren's Medical Center Research Institute, University of Texas Southwestern Medical Center, USA.
Xiaodong WenAdvanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Ian R CorbinAdvanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
A Dean SherryAdvanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Radiology, University of Texas Southwestern Medical Center, USA; Department of Chemistry, University of Texas at Dallas, Richardson, TX 75080, USA.
Craig R MalloyAdvanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Radiology, University of Texas Southwestern Medical Center, USA; Department of Internal Medicine, University of Texas Southwestern Medical Center, USA; VA North Texas Health Care System, Dallas, TX 75216, USA.
Eunsook S JinAdvanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Internal Medicine, University of Texas Southwestern Medical Center, USA. Electronic address: Eunsook.Jin@utsouthwestern.edu.
Southwestern Medical Center · USThe University of Texas Southwestern Medical Center · USAdvanced Imaging Research (United States) · USThe University of Texas at Dallas · USVA North Texas Health Care System · US

Funding

TARGETED RESPONSIVE MR AND PET AGENTS FOR B-CELL IMAGINGP01DK058398 · NIDDK · UNIVERSITY OF TEXAS SW MED CTR/DALLAS · PI MUOIO, DEBORAH M · 2000 to 2016
$26.1M
TR&D 3: Imaging Carbohydrate MetabolismP41EB015908 · NIBIB · UT SOUTHWESTERN MEDICAL CENTER · PI MALLOY, CRAIG R · 2012 to 2021
$10.9M
13C asymmetry in blood glucose as a marker for oxidative stress in liverR01DK099289 · NIDDK · UT SOUTHWESTERN MEDICAL CENTER · PI JIN, EUNSOOK · 2014 to 2017
$1.1M
NIBIB NIH HHS P41 EB015908NIDDK NIH HHS P01 DK058398NIDDK NIH HHS R01 DK099289
6 · The paper itself

Abstract

backgroundTherapies targeting altered activity of pyruvate dehydrogenase (PDH) and pyruvate carboxylase (PC) have been proposed for hepatomas. However, the activities of these pathways in hepatomas in vivo have not been distinguished. Here we examined pyruvate entry into the tricarboxylic acid (TCA) cycle through PDH versus PC in vivo using hepatoma-bearing rats.

methodsHepatoma-bearing rats were generated by intrahepatic injection of H4IIE cells. Metabolism of

resultsIn orthotopic hepatomas, pyruvate entry into the TCA cycle occurred exclusively through PDH and the excess PDH activity compared to normal liver was attributed to downregulated pyruvate dehydrogenase kinase (PDK) 2/4. However, pyruvate carboxylation via PC and gluconeogenesis were minimal, which was linked to downregulated forkhead box O1 (FoxO1) by Akt activity. In contrast to many studies of cancer metabolism, lactate production in hepatomas was not increased which corresponded to reduced expression of lactate dehydrogenase. The production of serine and glycine in hepatomas was enhanced, but glycine decarboxylase was downregulated.

conclusionsThe combination of [U-

Indexed as

GluconeogenesisAnimalsCarbon-13 Magnetic Resonance SpectroscopyCarcinoma, HepatocellularCitric Acid CycleGlycerolLiverLiver NeoplasmsPyruvate Dehydrogenase ComplexRatsGlycerolPyruvate Dehydrogenase ComplexCancer metabolismGlycerolHepatocellular carcinomaPyruvate carboxylasePyruvate dehydrogenaseTricarboxylic acid cycle

Identifiers

PMID31672442
PMCPMC6892165
OpenAlexW2982608757

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.