Evidence map›Paper›PMID 31671709›Full record

ArticleInternational journal of environmental research and public health2019

Comparison of Culture- and Quantitative PCR-Based Indicators of Antibiotic Resistance in Wastewater, Recycled Water, and Tap Water.

Jaqueline Rocha, Telma Fernandes, Maria V Riquelme, Ni Zhu, Amy Pruden, Célia M Manaia

Open access · goldAbstract read
In one paragraph

Article in International journal of environmental research and public health, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Comparison of Cefotaxime-ResistantAntibiotics (Basel, Switzerland) · 2023
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Markers Specific toInternational journal of environmental research and public health · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Jaqueline RochaCBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal. jrocha@porto.ucp.pt.
Telma FernandesCBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal. telma.7919@gmail.com.
Maria V RiquelmeDepartment of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA 24061, USA. mvprieto@vt.edu.
Ni ZhuDepartment of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA 24061, USA. niz@vt.edu.
Amy PrudenDepartment of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA 24061, USA. apruden@vt.edu.
Célia M ManaiaCBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal. cmanaia@porto.ucp.pt.ORCID 0000-0002-3674-1789
Universidade Católica Portuguesa · PTVirginia Tech · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Standardized methods are needed to support monitoring of antibiotic resistance in environmental samples. Culture-based methods target species of human-health relevance, while the direct quantification of antibiotic resistance genes (ARGs) measures the antibiotic resistance potential in the microbial community. This study compared measurements of tetracycline-, sulphonamide-, and cefotaxime-resistant presumptive total and fecal coliforms and presumptive enterococci versus a suite of ARGs quantified by quantitative polymerase chain reaction (qPCR) across waste-, recycled-, tap-, and freshwater. Cross-laboratory comparison of results involved measurements on samples collected and analysed in the US and Portugal. The same DNA extracts analysed in the US and Portugal produced comparable qPCR results (variation <28%), except for

Indexed as

Genes, BacterialAnti-Bacterial AgentsDrinking WaterDrug Resistance, MicrobialHumansPolymerase Chain ReactionPortugalRecyclingUnited StatesWastewaterWaterWater MicrobiologyAnti-Bacterial AgentsDrinking WaterWastewaterWaterantibiotic resistance monitoringantibiotic resistant coliformswater quality

Identifiers

PMID31671709
PMCPMC6862664
OpenAlexW2982708289

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.