ArticleWater research2020
Droplet digital PCR quantification of norovirus and adenovirus in decentralized wastewater and graywater collections: Implications for onsite reuse.
Article in Water research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Towards ultra-sensitive and rapid near-source wastewater-based epidemiology.Nature communications · 2025Article
- Digital PCR in Virology: Current Applications and Future Perspectives.Molecular diagnosis & therapy · 2025Review
- Validating digital polymerase chain reaction for 16S rRNA gene amplification from low biomass environmental samples.ISME communications · 2025Article
- The effect of sewage source on HF183 risk-based threshold estimation for recreational water quality management.Microbial risk analysis · 2024Article
- Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings.Diagnostics (Basel, Switzerland) · 2024Review
- Viruses in Wastewater-A Concern for Public Health and the Environment.Microorganisms · 2024Review
- Evaluating endogenous viral targets as potential treatment monitoring surrogates for onsite non-potable water reuse.Environmental science : water research & technology · 2024Article
- Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples.FEMS microbes · 2024Article
- Risk assessment of norovirus and hepatitis A virus in strawberries imported into China.Food science & nutrition · 2023Article
- SARS-CoV-2 detection and inactivation in water and wastewater: review on analytical methods, limitations and future research recommendations.Emerging microbes & infections · 2023Review
- Enteric pathogen reduction targets for onsite non-potable water systems: A critical evaluation.Water research · 2023Review
- Evaluation of intra- and inter-lab variability in quantifying SARS-CoV-2 in a state-wide wastewater monitoring network.Environmental science : water research & technology · 2023Article
- Geospatial Patterns of Antimicrobial Resistance Genes in the US EPA National Rivers and Streams Assessment Survey.Environmental science & technology · 2022Article
- Characterization of roof runoff microbial quality in four U.S. cities with varying climate and land use characteristics.Water research · 2022Article
- Wastewater surveillance for SARS-CoV-2 to support return to campus: Methodological considerations and data interpretation.Current opinion in environmental science & health · 2022Review
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
- SARS-CoV-2 monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data.The Science of the total environment · 2022Article
- Article
- Global public health implications of human exposure to viral contaminated water.Frontiers in microbiology · 2022Review
- Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review.Frontiers of environmental science & engineering · 2022Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Risk-based treatment of onsite wastewaters for decentralized reuse requires information on the occurrence and density of pathogens in source waters, which differ from municipal wastewater due to scaling and dilution effects in addition to variable source contributions. In this first quantitative report of viral enteric pathogens in onsite-collected graywater and wastewater, untreated graywater (n = 50 samples) and combined wastewater (i.e., including blackwater; n = 28) from three decentralized collection systems were analyzed for two norovirus genogroups (GI/GII) and human adenoviruses using droplet digital polymerase chain reaction (ddPCR). Compared to traditional quantitative PCR (qPCR), which had insufficient sensitivity to quantify viruses in graywater, ddPCR allowed quantification of norovirus GII and adenovirus in 4% and 14% of graywater samples, respectively (none quantifiable for norovirus GI). Norovirus GII was routinely quantifiable in combined wastewater by either PCR method (96% of samples), with well-correlated results between the analyses (R
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