ArticlePloS one2019
Evaluation of protocols for rRNA depletion-based RNA sequencing of nanogram inputs of mammalian total RNA.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Review
- Low-volumeMicrobial genomics · 2025Article
- The APOE isoforms differentially shape the transcriptomic and epigenomic landscapes of human microglia xenografted into a mouse model of Alzheimer's disease.Nature communications · 2025Article
- Exploring the influence of pre-analytical variables on gene expression measurements and relative expression orderings in cancer research.Scientific reports · 2025Article
- A scalable and cost-efficient rRNA depletion approach to enrich RNAs for molecular biology investigations.RNA (New York, N.Y.) · 2024Article
- Adaptable and comprehensive approaches for long-read nanopore sequencing of polyadenylated and non-polyadenylated RNAs.Frontiers in genetics · 2024Article
- Challenges and opportunities to computationally deconvolve heterogeneous tissue with varying cell sizes using single-cell RNA-sequencing datasets.Genome biology · 2023Review
- Performance of computational algorithms to deconvolve heterogeneous bulk ovarian tumor tissue depends on experimental factors.Genome biology · 2023Article
- Article
- Review
- RNA-seq RNAaccess identified as the preferred method for gene expression analysis of low quality FFPE samples.PloS one · 2023Article
- Library adaptors with integrated reference controls improve the accuracy and reliability of nanopore sequencing.Nature communications · 2022Article
- A method for simultaneous detection of small and long RNA biotypes by ribodepleted RNA-Seq.Scientific reports · 2022Article
- A Scalable Strand-Specific Protocol Enabling Full-Length Total RNA Sequencing From Single Cells.Frontiers in genetics · 2021Article
- A smart polymer for sequence-selective binding, pulldown, and release of DNA targets.Communications biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors.
Funding
Abstract
Next generation RNA-sequencing (RNA-seq) is a flexible approach that can be applied to a range of applications including global quantification of transcript expression, the characterization of RNA structure such as splicing patterns and profiling of expressed mutations. Many RNA-seq protocols require up to microgram levels of total RNA input amounts to generate high quality data, and thus remain impractical for the limited starting material amounts typically obtained from rare cell populations, such as those from early developmental stages or from laser micro-dissected clinical samples. Here, we present an assessment of the contemporary ribosomal RNA depletion-based protocols, and identify those that are suitable for inputs as low as 1-10 ng of intact total RNA and 100-500 ng of partially degraded RNA from formalin-fixed paraffin-embedded tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.