Evidence map›Paper›PMID 31670846›Full record

ArticleJournal of forensic sciences2020

Sensitive Method for the Confident Identification of Genetically Variant Peptides in Human Hair Keratin.

Zheng Zhang, Meghan C Burke, William E Wallace, Yuxue Liang, Sergey L Sheetlin, Yuri A Mirokhin, Dmitrii V Tchekhovskoi, Stephen E Stein

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of forensic sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Article
  3. DNA and protein analyses of hair in forensic genetics.International journal of legal medicine · 2023
    Review
  4. Identifying individuals using proteomics: are we there yet?Frontiers in molecular biosciences · 2022
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zheng ZhangBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.ORCID https://orcid.org/0000-0001-7058-4011
Meghan C BurkeBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
William E WallaceBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
Yuxue LiangBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
Sergey L SheetlinBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
Yuri A MirokhinBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
Dmitrii V TchekhovskoiBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
Stephen E SteinBiomolecular Measurement Division, Mass Spectrometry Data Center, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899.
National Institute of Standards and Technology · US

Funding

Intramural NIST DOC 9999-NISTNIST Special Programs Office
6 · The paper itself

Abstract

Recent reports have demonstrated that genetically variant peptides derived from human hair shaft proteins can be used to differentiate individuals of different biogeographic origins. We report a method involving direct extraction of hair shaft proteins more sensitive than previously published methods regarding GVP detection. It involves one step for protein extraction and was found to provide reproducible results. A detailed proteomic analysis of this data is presented that led to the following four results: (i) A peptide spectral library was created and made available for download. It contains all identified peptides from this work, including GVPs that, when appropriately expanded with diverse hair-derived peptides, can provide a routine, reliable, and sensitive means of analyzing hair digests; (ii) an analysis of artifact peptides arising from side reactions is also made using a new method for finding unexpected modifications; (iii) detailed analysis of the gel-based method employed clearly shows the high degree of cross-linking or protein association involved in hair digestion, with major GVPs eluting over a wide range of high molecular weights while others apparently arise from distinct non-cross-linked proteins; and (v) finally, we show that some of the specific GVP identifications depend on the sample preparation method.

Indexed as

ArtifactsChromatography, LiquidDatabases, ProteinForensic MedicineHairHumansKeratins, Hair-SpecificMaleMass SpectrometryPeptidesProteomeProteomicsReproducibility of ResultsKeratins, Hair-SpecificPeptidesProteomecuticular keratinsforensic sciencegenetically variant peptidehair protein extractionpeptide mass spectral librarytrace detection

Identifiers

PMID31670846
PMCPMC7064992
OpenAlexW2988286394

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.