ReviewBone2020
Development of protocols for the first serial block-face scanning electron microscopy (SBF SEM) studies of bone tissue.
Review in Bone, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Tubule and microbranch variations in human dentine: A quantitative 3D study with serial block-face scanning electron microscopy.Journal of microscopy · 2026Article
- Integrating serial block-face SEM with voxel-based finite element analysis for high-fidelity micromechanical modelling of anisotropic soft tissues: application to human dermis.Biomechanics and modeling in mechanobiology · 2026Article
- Three-dimensional visualization of the cytotrophoblast layer of human term chorionic villi.Histochemistry and cell biology · 2025Article
- Development, characterization, and curve fitting of rate-dependent models of calcified cerebral embolus analogs for acute ischemic stroke.Biomechanics and modeling in mechanobiology · 2025Article
- New Approaches Based on Serial-Block Face Electron Microscopy to Investigate the Peripheral Nervous System.Journal of the peripheral nervous system : JPNS · 2025Article
- Image analysis of kimchi cabbage penetrated with brine and seasoning using a serial block face scanning electron microscope and energy dispersive X-ray spectroscopy.Food science and biotechnology · 2025Article
- Review
- The desmosome comes into focus.The Journal of cell biology · 2024Review
- 2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS).Current osteoporosis reports · 2024Review
- Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers.PloS one · 2024Article
- Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest.Frontiers in zoology · 2023Article
- Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology.Advanced biology · 2023Review
- Contributions of Resin Cast Etching to Visualising the Osteocyte Lacuno-Canalicular Network Architecture in Bone Biology and Tissue Engineering.Calcified tissue international · 2023Review
- Modern Microscopic Approaches to Astrocytes.International journal of molecular sciences · 2023Review
- Review
- Endometrial gland specific progestagen-associated endometrial protein and cilia gene splicing changes in recurrent pregnancy loss.Reproduction & fertility · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Article
- Article
- Field-Emission Scanning Electron Microscope as a Tool for Large-Area and Large-Volume Ultrastructural Studies.Animals : an open access journal from MDPI · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
There is an unmet need for a high-resolution three-dimensional (3D) technique to simultaneously image osteocytes and the matrix in which these cells reside. In serial block-face scanning electron microscopy (SBF SEM), an ultramicrotome mounted within the vacuum chamber of a microscope repeatedly sections a resin-embedded block of tissue. Backscattered electron scans of the block face provide a stack of high-resolution two-dimensional images, which can be used to visualise and quantify cells and organelles in 3D. High-resolution 3D images of biological tissues from SBF SEM have been exploited considerably to date in the neuroscience field. However, non-brain samples, in particular hard biological tissues, have appeared more challenging to image by SBF SEM due to the difficulties of sectioning and rendering the samples conductive. We have developed and propose protocols for bone tissue preparation using SBF SEM, for imaging simultaneously soft and hard bone tissue components in 3D. We review the state of the art in high-resolution imaging of osteocytes, provide a historical perspective of SBF SEM, and we present first SBF SEM proof-of-concept studies for murine and human tissue. The application of SBF SEM to hard tissues will facilitate qualitative and quantitative 3D studies of tissue microstructure and ultrastructure in bone development, ageing and pathologies such as osteoporosis and osteoarthritis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.