Evidence map›Paper›PMID 31668229›Full record

ArticleMethods in enzymology2019

Synthetic probe development for measuring single or few-cell activity and efflux.

Alison Lui, Jeffrey Wang, Linda Chio, Markita P Landry

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alison LuiDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, United States.
Jeffrey WangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, United States.
Linda ChioDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, United States.
Markita P LandryDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, United States; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA, United States; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, United States; California Institute for Quantitative Biosciences (QB3), Berkeley, CA, United States; Chan-Zuckerberg Biohub, San Francisco, CA, United States. Electronic address: landry@berkeley.edu.

Funding

Single-Molecule Imaging of Biological Trauma:Cytokine-Based Intracellular CommunicationR35GM128922 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI LANDRY, MARKITA · 2019 to 2023
$2.0M
Novel optical probe for dopamine release in neural circuitsR21EY029405 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI FELLER, MARLA, LANDRY, MARKITA · 2018 to 2019
$428k
NEI NIH HHS R21 EY029405NIGMS NIH HHS R35 GM128922
6 · The paper itself

Abstract

Studying the single cell protein secretome offers the opportunity to understand how a phenotypically heterogeneous population of individual cells contribute to ensemble physiology and signaling. Polarized secretion events such as neurotransmitter release and cytokine signaling necessitates spatiotemporal information to elucidate structure-function relationships. Polymer functionalized single-walled carbon nanotube protein sensor arrays allow microscopic imaging of secreted protein footprints and enable the study of the spatiotemporal heterogeneity of protein secretion at the single-cell level. The protocols for carbon nanotube sensor creation, sensor array preparation, and imaging secreted proteins in both prokaryotic and mammalian cells are presented in this chapter. Secreted RAP1 and HIV-1 integrase proteins were used as proof-of-concept examples. Additionally, we discuss potential variety of protein and non-protein analyte effluxes that can be imaged using this platform, as well as current and future perspectives related to sensor development and deployment.

Indexed as

AnimalsAptamers, NucleotideBiosensing TechniquesCell LineEquipment DesignHumansMicrofluidic Analytical TechniquesMicroscopy, FluorescenceNanotubes, CarbonOptical ImagingPolymersProteinsSingle-Cell AnalysisAptamers, NucleotideNanotubes, CarbonPolymersProteinsAmphiphilic heteropolymer sensorAptamerCellular metabolomeSecretomeSingle-cell spectroscopySingle-walled carbon nanotubeSurface array

Identifiers

PMID31668229
PMCPMC10461879

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.