ArticleEMBO reports2019
Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation.
Article in EMBO reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Optimized optogenetic anti-CRISPR for endogenous gene regulation in Drosophila.Nucleic acids research · 2026Article
- Neuralized-like proteins differentially activate Notch ligands.EMBO reports · 2025Article
- The intracellular domains of the DSL ligands Serrate and Delta provide different activities.Cell communication and signaling : CCS · 2025Article
- Article
- Differential regulation of the proteome and phosphoproteome along the dorso-ventral axis of the earlyeLife · 2024Article
- The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila.BMC biology · 2023Article
- Synthetic developmental biology: New tools to deconstruct and rebuild developmental systems.Seminars in cell & developmental biology · 2023Review
- Mechanochemical feedback loops in contact-dependent fate patterning.Current opinion in systems biology · 2023Review
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Optogenetic control of YAP cellular localisation and function.EMBO reports · 2022Article
- SALSA, a genetically encoded biosensor for spatiotemporal quantification of Notch signal transduction in vivo.Developmental cell · 2022Article
- Revealing epithelial morphogenetic mechanisms through live imaging.Current opinion in genetics & development · 2022Review
- Rapid and robust optogenetic control of gene expression in Drosophila.Developmental cell · 2021Article
- Mechanical competition alters the cellular interpretation of an endogenous genetic program.The Journal of cell biology · 2021Article
- Membrane architecture and adherens junctions contribute to strong Notch pathway activation.Development (Cambridge, England) · 2021Article
- Desensitisation of Notch signalling through dynamic adaptation in the nucleus.The EMBO journal · 2021Article
- Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos.Developmental cell · 2021Article
- Optogenetic approaches for understanding homeostatic and degenerative processes in Drosophila.Cellular and molecular life sciences : CMLS · 2021Review
- Signaling, Deconstructed: Using Optogenetics to Dissect and Direct Information Flow in Biological Systems.Annual review of biomedical engineering · 2021Review
- Biophysics of Notch Signaling.Annual review of biophysics · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatio-temporal regulation of signalling pathways plays a key role in generating diverse responses during the development of multicellular organisms. The role of signal dynamics in transferring signalling information in vivo is incompletely understood. Here, we employ genome engineering in Drosophila melanogaster to generate a functional optogenetic allele of the Notch ligand Delta (opto-Delta), which replaces both copies of the endogenous wild-type locus. Using clonal analysis, we show that optogenetic activation blocks Notch activation through cis-inhibition in signal-receiving cells. Signal perturbation in combination with quantitative analysis of a live transcriptional reporter of Notch pathway activity reveals differential tissue- and cell-scale regulatory modes. While at the tissue-level the duration of Notch signalling determines the probability with which a cellular response will occur, in individual cells Notch activation acts through a switch-like mechanism. Thus, time confers regulatory properties to Notch signalling that exhibit integrative digital behaviours during tissue differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.