Evidence map›Paper›PMID 31667801›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2020

A novel Trichoderma reesei mutant RP698 with enhanced cellulase production.

Jean Carlos Rodrigues Silva, José Carlos Santos Salgado, Ana Claudia Vici, Richard John Ward, Maria Lourdes Teixeira Moraes Polizeli, Luis Henrique Souza Guimarães, Rosa Prazeres Melo Furriel, João Atílio Jorge

Open access · greenAbstract read
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 27 citations in OpenAlex.

  1. Simultaneous Biosynthesis of Cellulase byWaste and biomass valorization · 2026
    Article
  2. Article
  3. Optimization and Bioreactor Scale-Up of Cellulase Production inInternational journal of molecular sciences · 2025
    Article
  4. Article
  5. Article
  6. Review
  7. A Combined Study on Optimization,Biochemistry research international · 2022
    Review
  8. Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jean Carlos Rodrigues SilvaDepartamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14049-900, São Paulo, Brazil.
José Carlos Santos SalgadoDepartamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil. salgado@usp.br.ORCID http://orcid.org/0000-0002-3422-9474
Ana Claudia ViciDepartamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil.
Richard John WardDepartamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil.
Maria Lourdes Teixeira Moraes PolizeliDepartamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil.
Luis Henrique Souza GuimarãesDepartamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil.
Rosa Prazeres Melo FurrielDepartamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil.
João Atílio JorgeDepartamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, 14040-901, São Paulo, Brazil.
Universidade de Ribeirão Preto · BRFederal Institute of São Paulo · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 305788/2017-5Conselho Nacional de Desenvolvimento Científico e Tecnológico 465319/2014-9Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.141280/2017-00Fundação de Amparo à Pesquisa do Estado de São Paulo 08/53249-8Fundação de Amparo à Pesquisa do Estado de São Paulo 08/58482-2Fundação de Amparo à Pesquisa do Estado de São Paulo 14/14415-0
6 · The paper itself

Abstract

A new strain of Trichoderma reesei (teleomorph Hypocrea jecorina) with high cellulase production was obtained by exposing the spores from T. reesei QM9414 to an ultraviolet light followed by selecting fast-growing colonies on plates containing CMC (1% w/v) as the carbon source. The mutant T. reesei RP698 reduced cultivation period to 5 days and increased tolerance to the end-products of enzymatic cellulose digestion. Under submerged fermentation conditions, FPase, CMCase, and Avicelase production increased up to 2-fold as compared to the original QM9414 strain. The highest levels of cellulase activity were obtained at 27 °C after 72 h with Avicel®, cellobiose, and sugarcane bagasse as carbon sources. The temperature and pH activity optima of the FPase, CMCase, and Avicelase were approximately 60 °C and 5.0, respectively. The cellulase activity was unaffected by the addition of 140 mM glucose in the enzyme assay. When T. reesei RP698 crude extract was supplemented by the addition of β-glucosidase from Scytalidium thermophilum, a 2.3-fold increase in glucose release was observed, confirming the low inhibition by the end-product of cellulose hydrolysis. These features indicate the utility of this mutant strain in the production of enzymatic cocktails for biomass degradation.

Indexed as

FermentationBiomassCellulaseFungal ProteinsHydrolysisHypocrealesMutationSaccharumUltraviolet RaysCellulaseFungal ProteinsCellulase productionCellulose hydrolysisCMCaseMutationTrichoderma reesei

Identifiers

PMID31667801
PMCPMC7203315
OpenAlexW2982357318

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.