Evidence map›Paper›PMID 31667713›Full record

ArticleMolecular biotechnology2020

Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation.

C Sunisha, H D Sowmya, T R Usharani, M Umesha, H R Gopalkrishna, Arvindkumar Saxena

Abstract read
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Article in Molecular biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Banana breeding by genome design.Journal of integrative plant biology · 2025
    Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

C SunishaDepartment of Biotechnology and Biochemistry, Centre for Post-Graduate Studies, Jain University, Bangalore, India.ORCID https://orcid.org/0000-0002-9908-5870
H D SowmyaDivision of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bangalore, 560 089, India.ORCID https://orcid.org/0000-0002-3213-3735
T R UsharaniDivision of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bangalore, 560 089, India. usharani.tr@icar.gov.in.
M UmeshaDivision of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bangalore, 560 089, India.ORCID https://orcid.org/0000-0002-5907-6224
H R GopalkrishnaDivision of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bangalore, 560 089, India.ORCID https://orcid.org/0000-0003-3679-3359
Arvindkumar SaxenaDivision of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bangalore, 560 089, India.ORCID https://orcid.org/0000-0003-4889-0981

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enhanced tolerance to wilt disease (Fusarium oxysporum f.sp. cubense) was achieved in banana variety Rasthali (AAB) by the transformation of embryogenic cells with two antimicrobial genes viz., Ace-AMP1 and pflp using Agrobacterium mediated transformation. The transgene copy numbers in stable transformants were confirmed by Southern analysis. The expression of stacked genes in the transgenic lines was validated by RT-PCR as well as Northern analysis. Bioassay using Foc race 1 in pot culture experiments demonstrated enhanced tolerance after 180 days of planting. Two independent transformants showed 10-20% Vascular Discoloration Index compared to untransformed banana cv. Rasthali (96%). The stacked lines revealed higher activity of Super Oxide Dismutase and Peroxidase compared to untransformed control which depicted higher tolerance to oxidative stress caused by Foc infection.

Indexed as

Agrobacterium tumefaciensAnti-Bacterial AgentsAnti-Infective AgentsFerredoxinsFusariumGene Expression ProfilingMusaOxidative StressPeroxidasePlant DiseasesPlant ProteinsPlant RootsPlants, Genetically ModifiedSuperoxide DismutaseTransformation, GeneticAMP1 protein, Allium cepaAnti-Bacterial AgentsAnti-Infective AgentsFerredoxinsPeroxidaseplant ferredoxin-like protein, Capsicum annuumPlant ProteinsSuperoxide DismutaseAntimicrobial peptideFusarium oxysporum f.sp. cubenseFusarium wiltPlant ferredoxin-like proteinRasthali

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.