Evidence map›Paper›PMID 31667700›Full record

ArticleJournal of assisted reproduction and genetics2020

Capacitation IVM improves cumulus function and oocyte quality in minimally stimulated mice.

Y Zhao, X Liao, A E Krysta, M J Bertoldo, D Richani, R B Gilchrist

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In one paragraph

Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
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  15. Effect of cumulin and super-GDF9 in standard and biphasic mouse IVM.Journal of assisted reproduction and genetics · 2022
    Article
  16. Review
  17. Review
  18. Review
  19. Article
  20. Increased Environment-Related Metabolism and Genetic Expression in theFrontiers in cell and developmental biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Y ZhaoReproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.ORCID http://orcid.org/0000-0001-5630-5805
X LiaoSchool of Women's & Children's Health, University of New South Wales, Sydney, NSW, 2052, Australia.
A E KrystaSchool of Women's & Children's Health, University of New South Wales, Sydney, NSW, 2052, Australia.
M J BertoldoSchool of Women's & Children's Health, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID http://orcid.org/0000-0002-9471-501X
D RichaniSchool of Women's & Children's Health, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID http://orcid.org/0000-0003-1886-0423
R B GilchristSchool of Women's & Children's Health, University of New South Wales, Sydney, NSW, 2052, Australia. r.gilchrist@unsw.edu.au.ORCID http://orcid.org/0000-0003-1611-7142

Funding

Fujian Provincial Health Commission Overseas Study ScholarshipStrategic Funds from the University of New South Wales Sydney APP1023210, APP1117538the National Health and Medical Research Council of Australia APP1076004, APP1062762, APP1139763Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (CN) Overseas Training Funding
6 · The paper itself

Abstract

purposeOocyte in vitro maturation (IVM) is a patient-friendly reproductive technology but lower success rates than IVF have limited its uptake. Capacitation-IVM (CAPA-IVM) is an innovative new IVM system currently undergoing clinical evaluation. This study aimed to determine temporal effects of the pre-IVM phase of CAPA-IVM on cumulus function and oocyte developmental competence in mildly-stimulated mice.

methodsImmature cumulus oocyte complexes (COCs) derived from mildly stimulated (23 h PMSG) 28-day-old mice underwent pre-IVM for 0-24 h in medium containing c-type natriuretic peptide (CNP), E

resultsDay 6 blastocyst rate increased incrementally with increasing pre-IVM duration: 40.6 ± 2.0%, 45.8 ± 1.2%, 52.2 ± 3.5%, 53.3 ± 5.9%, and 59.9 ± 2.5% for 0, 2, 6, 12, and 24 h pre-IVM, respectively (P < 0.01). DNA content/COC, a measure of cumulus cell proliferation, was significantly higher with 24 h pre-IVM group compared to 0, 2, or 6 h pre-IVM (P < 0.001). Pre-IVM for 24 h significantly increased cumulus expansion and mRNA expression of matrix genes Has2 and Tnfaip6 and Areg relative to no pre-IVM control (P < 0.01). Cumulus-oocyte gap-junctional communication (GJC) was maintained throughout 24 h pre-IVM (P < 0.0001), and GJC loss was slowed during the subsequent IVM phase, whilst meiotic resumption was accelerated (P < 0.05). Pre-IVM increased COC ATP and ADP content (P < 0.05), but not AMP, ATP/ADP, and energy charge.

conclusionThe pre-IVM phase of CAPA-IVM improves the quality of IVM oocytes in a temporally dependent manner and significantly influences cumulus cell function including increased cell proliferation, cumulus expansion, and prolonged cumulus-oocyte GJC.

Indexed as

Embryonic DevelopmentMeiosisOogenesisAnimalsCumulus CellsFemaleIn Vitro Oocyte Maturation TechniquesMiceMice, Inbred C57BLOocytesCapacitation-IVM (CAPA-IVM)c-type natriuretic peptide (CNP)Cumulus-oocyte gap junction communicationOocyte in vitro maturation (IVM)Oocyte qualityPre-maturation (pre-IVM)

Identifiers

PMID31667700
PMCPMC7000615

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.