Evidence map›Paper›PMID 31667558›Full record

ArticleEuropean biophysics journal : EBJ2019

Experimental determination of second virial coefficients by small-angle X-ray scattering: a problem revisited.

Tyler Mrozowich, Donald J Winzor, David J Scott, Trushar R Patel

Abstract read
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In one paragraph

Article in European biophysics journal : EBJ, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tyler MrozowichDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Drive, Lethbridge, AB, T1K 3M4, Canada.ORCID http://orcid.org/0000-0002-1077-9682
Donald J WinzorSchool of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, 4072, Australia.
David J ScottNational Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Nottingham, LE12 5RD, UK. david.scott@nottingham.ac.uk.ORCID http://orcid.org/0000-0002-6726-2078
Trushar R PatelDepartment of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Drive, Lethbridge, AB, T1K 3M4, Canada. trushar.patel@uleth.ca.ORCID http://orcid.org/0000-0003-0627-2923

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This investigation examines the validity of employing single-solute theory to interpret SAXS measurements on buffered protein solutions-the current practice despite the necessity to regard the buffer components as additional non-scattering solutes rather than as part of the solvent. The present study of bovine serum albumin in phosphate-buffered saline supplemented with 20-100 g/L sucrose as small cosolute has certainly verified the prediction that the experimentally obtained second virial coefficient should contain protein-cosolute contributions. Nevertheless, the second virial coefficient determined for protein solutions supplemented with high cosolute concentrations on the basis of single-solute theory remains a valid means for identifying conditions conducive to protein crystallization, because the return of a slightly negative second virial coefficient based on single-solute theory [Formula: see text] still establishes the existence of slightly associative interactions between protein molecules, irrespective of the molecular source-protein self-interactions and/or protein-cosolute contributions.

Indexed as

Scattering, Small AngleAnimalsCattleProtein BindingSerum Albumin, BovineX-Ray DiffractionSerum Albumin, BovineBovine serum albuminSecond virial coefficientsSmall-angle X-ray scatteringSmall cosolute effectsThermodynamic nonideality

Identifiers

PMID31667558

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.