Evidence map›Paper›PMID 31667436›Full record

ArticleHeliyon2019

Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway.

Hirotoshio Motoda, Fukumi Hiragami, Kenji Kawamura, Shigeki Inoue, Yutaka Gomita, Yoshio Kano

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Hirotoshio MotodaGraduate School of Health Science, Kibi International University, 8-Iga-machi, Takahashi, Okayama, 716-8508, Japan.
Fukumi HiragamiGraduate School of Health Science, Kibi International University, 8-Iga-machi, Takahashi, Okayama, 716-8508, Japan.
Kenji KawamuraGraduate School of Health Science, Kibi International University, 8-Iga-machi, Takahashi, Okayama, 716-8508, Japan.
Shigeki InoueGraduate School of Health Science, Kibi International University, 8-Iga-machi, Takahashi, Okayama, 716-8508, Japan.
Yutaka GomitaResearch Institute of Health and Welfare, Kibi International University, 8-Iga-machi, Takahashi, Okayama, 716-8508, Japan.
Yoshio KanoGraduate School of Health Science, Kibi International University, 8-Iga-machi, Takahashi, Okayama, 716-8508, Japan.
Kibi International University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigated the differentiation and activation of p38 MAPK induced by contrast bath in drug-hypersensitive PC12m3 mutant cells. The rate of neurite outgrowth in PC12m3 cells induced by contrast bath was much higher than that induced by warming or cooling alone or that induced by two warmings with an interval of room temperature, indicating that contrast bath has a synergistic effect. The results of an experiment using a p38 MAPK inhibitor, SB203580, showed that neurite outgrowth of PC12m3 cells induced by contrast bath is p38 MAPK-dependent. Moreover, p38 MAPK activity induced by contrast bath was greater than that induced by warming or cooling alone, indicating that the synergistic effect of a contrast bath on neurite outgrowth depends on the activity of p38 MAPK. Since calcium ions are involved in the activations of P38 MAPK, we investigated the effect of the TRP ion channel inhibitor (Capsazepine) that inhibits calcium influx in the cells. Neurite outgrowth induced by contrast bath treatment was greatly suppressed by the addition of Capsazepine. These findings suggest that calcium dependent activation of the p38 MAPK pathway induced by contrast bath is responsible for the neurite outgrowth of PC12m3 cells.

Indexed as

Cell biologyCell cultureCell differentiationContrast bathCoolingNeurite outgrowthNeurosciencep38 MAPKWarming

Identifiers

PMID31667436
PMCPMC6812228
OpenAlexW2981233853

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.