ArticleMethodsX2019
An alternative, easy and reproducible method of stabilization and ligature-induced periodontitis in mouse.
Article in MethodsX, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Oral Supplementation ofDentistry journal · 2026Article
- Human calculus adhesive-induced periodontitis model recapitulating human periodontitis microbiota in rats.BMC oral health · 2025Article
- Induction of Epithelial-Mesenchymal Transition in Periodontitis Rat Model.European journal of dentistry · 2025Article
- Preparation of Ag-Metal organic frameworks-loaded Sodium Alginate Hydrogel for the treatment of periodontitis.Scientific reports · 2025Article
- Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Attenuates Experimental Periodontitis in Mice Partly by Delivering miRNAs.International journal of nanomedicine · 2025Article
- Infrared laser therapy decreases systemic oxidative stress and inflammation in hypercholesterolemic mice with periodontitis.Lipids in health and disease · 2023Article
- A modified method for constructing experimental rat periodontitis model.Frontiers in bioengineering and biotechnology · 2022Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Periodontal disease is one of the most common causes of tooth loss in the world. Ligature-induced is the most used method to study periodontitis. Here, we describe a alternative, easy and accessible experimental technique of ligation in mice. Twenty C57BL/6 female mice were divided in two groups, control and ligation. Ligature group (n = 10) was immobilized in a well described stabilization board and ligature was performed at the first molar using a new procedure here described in detail. Eight weeks later animals were euthanized, and periodontitis hallmarks were evaluated. Ligatures remained attached to the teeth in all animal during the hole experiment. The procedure induced a temporary loss of weight but no causalities or tooth loss. The animals affected by ligation in their molar teeth presented all periodontitis hallmarks, including alveolar bone loss, gingival retraction and inflammatory infiltrate in the studied region both macro and microscopically. The alternative method is low cost, easily reproducible, and induces all periodontitis hallmarks that are sustained until 8 weeks after placement. •Ligature-induced periodontitis in mouse is a powerful tool of research.•Methods describing the procedure in literature are difficult to reproduce.•A alternative stabilization and ligation procedure in mice is completely described here.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.