ArticleMethodsX2019
Determination of conjugated protein on nanoparticles by an adaptation of the Coomassie blue dye method.
Article in MethodsX, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Co-amendment of rumen microorganism and bamboo biochar to optimize VFAs synthesis in cornstalk anaerobic fermentation: performance evaluation and mechanism understanding.Bioprocess and biosystems engineering · 2026Article
- Nanosystems Containing Proteins: From Fundamentals to Applications.Chemistry, an Asian journal · 2026Review
- Dual Potential of Cetuximab Conjugated Hydroxyapatite Zirconium Nanoparticle as Nanocarrier for Radioenhancer in X-Ray Dynamic Therapy andNanotheranostics · 2025Article
- Integrated insulin-iron nanoparticles: a multi-modal approach for receptor-specific bioimaging, reactive oxygen species scavenging, and wound healing.Discover nano · 2024Article
- A targeted fluorescent nanosensor for ratiometric pH sensing at the cell surface.Scientific reports · 2024Article
- Therapeutic potential of lactoferrin-coated iron oxide nanospheres for targeted hyperthermia in gastric cancer.Scientific reports · 2023Article
- Insulin-cobalt core-shell nanoparticles for receptor-targeted bioimaging and diabetic wound healing.RSC advances · 2023Article
- Antibody-Functionalized Copper Oxide Nanoparticles with Targeted Antibacterial Activity.ChemistryOpen · 2023Article
- The Oral Inactivated Porcine Epidemic Diarrhea Virus Presenting in the Intestine Induces Mucosal Immunity in Mice with Alginate-Chitosan Microcapsules.Animals : an open access journal from MDPI · 2023Article
- Enzyme Prodrug Therapy with Photo-Cross-Linkable Anti-EGFR Affibodies Conjugated to Upconverting Nanoparticles.ACS nano · 2022Article
- Characterization of GPVI- or GPVI-CD39-Coated Nanoparticles and Their Impact on In Vitro Thrombus Formation.International journal of molecular sciences · 2021Article
- Thin Film Plastic Antibody-Based Microplate Assay for Human Serum Albumin Determination.Polymers · 2021Article
- High Surface Reactivity and Biocompatibility of YMolecules (Basel, Switzerland) · 2020Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new accurate spectrophotometric method for protein determination on nanoparticles is described. The method is based on the Coomassie blue dye that binds to the basic and aromatic amino acid residues of proteins, especially arginine and lysine. A known amount of reagent dye was mixed with a variety of protein-loaded nanoparticles. Thereafter the unconjugated reagent was mixed with excess protein (bovine serum albumin) and titrated. In this method, the reacted dye on the protein coating of nanoparticle is directly determined, in opposite to the conventional method, in which the conjugated protein is determined as the difference between the non-conjugated protein found in the supernatant after centrifugation, and the total amount of protein originally used. This method is able to measure amounts of coated protein lower than 1 ppm. •Simple and accurate method especially adapted for protein-coated nanoparticles.•The amino acid residues of protein in the nanoparticle surface react with Coomassie brilliant blue dye.•The unreacted dye is titrated with an excess of a standard protein.
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