ArticleLight, science & applications2019
Simple and efficient delivery of cell-impermeable organic fluorescent probes into live cells for live-cell superresolution imaging.
Article in Light, science & applications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- Organelle-specific fluorescent probes for live imaging at the nanoscale.Science China. Life sciences · 2026Review
- Article
- Illuminating Mitochondrial Dynamics: Ultrahigh Labeling Stability Probe for Long-Term SIM Super-Resolution Imaging of Mitochondria.ACS central science · 2025Article
- Organic fluorescent probes for live-cell super-resolution imaging.Frontiers of optoelectronics · 2023Review
- Elucidating the Impact of Payload Conjugation on the Cell-Penetrating Efficiency of the Endosomal Escape Peptide dfTAT: Implications for Future Designs for CPP-Based Delivery Systems.Bioconjugate chemistry · 2023Article
- Three-dimensional multi-color optical nanoscopy at sub-10-nm resolution based on small-molecule organic probes.Cell reports methods · 2023Article
- Organic Anion Transporting Polypeptide 3A1 (OATP3A1)-Gated Bio-Orthogonal Labeling of Intracellular Proteins.Molecules (Basel, Switzerland) · 2023Article
- Giant proteins in a giant cell: Molecular basis of ultrafast CaScience advances · 2023Article
- Hydrophobicity is a key determinant in the activity of arginine-rich cell penetrating peptides.Scientific reports · 2022Article
- Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales.Nature methods · 2022Article
- Facile Labeling of Sieve Element Phloem-Protein Bodies Using the Reciprocal Oligosaccharide Probe OGAFrontiers in plant science · 2022Article
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Simultaneous ZnNature communications · 2021Article
- Fluorescent Probes for Super-Resolution Microscopy of Lysosomes.ACS omega · 2020Review
- Mechanism of Cell Penetration by Permeabilization of Late Endosomes: Interplay between a Multivalent TAT Peptide and Bis(monoacylglycero)phosphate.Cell chemical biology · 2020Article
- A Trojan Horse for live-cell super-resolution microscopy.Light, science & applications · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell permeability. Here, we develop a simple but effective strategy that renders cells permeable to cell-impermeable, organic fluorescent probes by using a novel peptide vehicle, PV-1. By simple coincubation with PV-1, 22 different cell-impermeable, organic fluorescent probes were efficiently delivered into live cells and specifically labeled a variety of organelles. Moreover, PV-1 can simultaneously transfer up to three different probes into live cells. By using PV-1 and these cell-impermeable fluorescent probes, we obtained multicolor, long-term, live-cell superresolution images of various organelles, which allowed us to study the dynamic interactions between them. PV-1, together with these organic fluorescent probes, will greatly broaden the applications of superresolution imaging technology in diverse live-cell studies and opens up a new avenue in the design and application of peptide vehicles.
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Registered trials
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