ArticlePlant methods2019
A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 76 citations in OpenAlex.
- Engineering plant tandem kinase immune receptors expands effector recognition profiles.Science advances · 2026Article
- No Cost of Resistance in Wheat Gene Stack Lines Containing 10 Stem Rust Resistance Transgenes.Plant biotechnology journal · 2026Article
- A transmembrane protein confers the Yr26/Yr24/YrCH42/YrG22-mediated stripe rust resistance in wheat.Nature communications · 2026Article
- A Rare Recombination in the Phytophthora sojae Genome Allows the Redefinition of a Major Avirulence Gene.Molecular plant pathology · 2026Article
- An HMA-like integrated domain in the wheat tandem kinase WTK4 recognises an RNase-like pathogen effector.Nature communications · 2026Article
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- Targeted capture and cloning of Rps6 reveal redundancy in soybean resistance genes.BMC plant biology · 2026Article
- Virulence on Pm4 kinase-based resistance is determined by two divergent wheat powdery mildew effectors.Nature plants · 2026Article
- ExPOSE: a comprehensive toolkit to perform expansion microscopy in plant protoplast systems.The Plant journal : for cell and molecular biology · 2025Article
- Resurrection of the Plant Immune Receptor Sr50 to Overcome Pathogen Immune Evasion.bioRxiv : the preprint server for biology · 2025Article
- A Wheat Protoplast Assay for Positive Effector Screening and Investigation of Host-Pathogen Interactions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The mutant STAY-GREEN (Molecular breeding : new strategies in plant improvement · 2024Article
- An array of Zymoseptoria tritici effectors suppress plant immune responses.Molecular plant pathology · 2024Article
- A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew.Nature communications · 2024Article
- Dissecting the causal polymorphism of the Lr67res multipathogen resistance gene.Journal of experimental botany · 2024Article
- Evolution of wheat blast resistance gene Rmg8 accompanied by differentiation of variants recognizing the powdery mildew fungus.Nature plants · 2024Article
- Physiochemical interaction between osmotic stress and a bacterial exometabolite promotes plant disease.Nature communications · 2024Article
- Pooled effector library screening in protoplasts rapidly identifies novel Avr genes.Nature plants · 2024Article
- MADS1-regulated lemma and awn development benefits barley yield.Nature communications · 2024Article
- A dominant-negative avirulence effector of the barley powdery mildew fungus provides mechanistic insight into barley MLA immune receptor activation.Journal of experimental botany · 2023Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant disease resistance to host-adapted pathogens is often mediated by host nucleotide-binding and leucine-rich repeat (NLR) receptors that detect matching pathogen avirulence effectors (AVR) inside plant cells. AVR-triggered NLR activation is typically associated with a rapid host cell death at sites of attempted infection and this response constitutes a widely used surrogate for NLR activation. However, it is challenging to assess this cell death in cereal hosts.
resultsHere we quantify cell death upon NLR-mediated recognition of fungal pathogen AVRs in mesophyll leaf protoplasts of barley and wheat. We provide measurements for the recognition of the fungal AVRs
conclusionsOur data demonstrate that the here described approach can be effectively used to detect and quantify death of wheat and barley cells induced by overexpression of
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