ArticleScientific reports2019
Detection and identification of transgenic events by next generation sequencing combined with enrichment technologies.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Multiplex PCR Fluorescence Method for Detection of Genetically Modified Maize Strains.Current issues in molecular biology · 2026Article
- Enhancing low-level genome-edited crop detection and identification in food mixtures using nanopore adaptive sampling: Rice-Soybean mixture as proof-of-concept.NPJ science of food · 2026Article
- A duplex sequencing approach for high-sensitivity detection of genome-edited plants.Food chemistry. Molecular sciences · 2025Article
- An integrated strategy involving high-throughput sequencing to characterize an unknown GM wheat event in Canada.Plant biotechnology journal · 2024Article
- Application of next-generation sequencing in the detection of transgenic crop.Frontiers in genetics · 2024Review
- Reproducibility of next-generation-sequencing-based analysis of a CRISPR/Cas9 genome edited oil seed rape.Food chemistry. Molecular sciences · 2023Article
- Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms.Foods (Basel, Switzerland) · 2023Article
- Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example.Food chemistry. Molecular sciences · 2022Article
- LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms.Plant biotechnology journal · 2022Article
- Transcriptomic Analysis of Mature Transgenic Poplar Expressing the Transcription FactorFrontiers in bioengineering and biotechnology · 2022Article
- Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer.Scientific reports · 2020Article
- Targeted MinION sequencing of transgenes.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Next generation sequencing (NGS) is a promising tool for analysing the quality and safety of food and feed products. The detection and identification of genetically modified organisms (GMOs) is complex, as the diversity of transgenic events and types of structural elements introduced in plants continue to increase. In this paper, we show how a strategy that combines enrichment technologies with NGS can be used to detect a large panel of structural elements and partially or completely reconstruct the new sequence inserted into the plant genome in a single analysis, even at low GMO percentages. The strategy of enriching sequences of interest makes the approach applicable even to mixed products, which was not possible before due to insufficient coverage of the different genomes present. This approach is also the first step towards a more complete characterisation of agrifood products in a single analysis.
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