ArticleProceedings of the National Academy of Sciences of the United States of America2019
Self-renewing endometrial epithelial organoids of the human uterus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers.
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Who cites it
151 citing papers in PubMed.
- Enzymatic cross-linking collagen-based bioink for 3D bioprinting of endometrial model with endometrial epithelial organoids.Bioactive materials · 2027Article
- Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Organoids: Current Applications and Future Directions.MedComm · 2026Review
- Review
- A non-invasive, patient-derived organoid platform from uterine lavage fluid for modeling endometrial implantation and disease.Stem cell research & therapy · 2026Article
- Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologies.STAR protocols · 2026Article
- Development and characterization of functional sheep endometrial luminal epithelial organoids.Veterinary research · 2026Article
- Organoid models: Regulations and prospects for the development of investigational new drugs.Chinese medical journal · 2026Review
- Eutopic echoes ectopic: organoid-based evidence of shared hormone dysregulation in endometriosis.Reproductive biology and endocrinology : RB&E · 2026Article
- Development of a three-dimensional culture model of endometrium to study embryo-uterine interactions.The Journal of reproduction and development · 2026Review
- Somatic mutations in endometrial epithelium: biological insights and emerging in vitro models.Molecular human reproduction · 2026Review
- Article
- Equine endometrial organoids preserve tissue structure and cycle-stage transcriptional identity†.Biology of reproduction · 2026Article
- Multi-Omics Data Reveal Estrogen-Driven Dysregulation and Stromal-Epithelial Signaling Alterations in Endometrial Polyps.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Engineering the Human Endometrial-Embryo Interface: Breakthroughs in 3D Uterine Models.Biomolecules · 2026Review
- Endometrial Assembloid Model Reveals Endometrial Gland Development Regulation by Estradiol-Driven WNT7B Suppression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Evolvoid: a genetic algorithm for shaping optimal cellular constructs.Journal of biological engineering · 2026Article
- Development and Transformation of Veterinary Experimental In Vitro Models: From 2D Culture to 3D Organoids.Animals : an open access journal from MDPI · 2026Review
- Single-cell mapping of human endometrium and decidua reveals epithelial and stromal contributions to fertility.JCI insight · 2026Article
- Embryo secretome regulation of the endometrial epithelial transcriptome: development of a model to test for embryo viability.Molecular human reproduction · 2026Article
91 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The human endometrium is essential in providing the site for implantation and maintaining the growth and survival of the conceptus. An unreceptive endometrium and disrupted maternal-conceptus interactions can cause infertility due to pregnancy loss or later pregnancy complications. Despite this, the role of uterine glands in first trimester human pregnancy is little understood. An established organoid protocol was used to generate and comprehensively analyze 3-dimensional endometrial epithelial organoid (EEO) cultures from human endometrial biopsies. The derived EEO expand long-term, are genetically stable, and can be cryopreserved. Using endometrium from 2 different donors, EEO were derived and then treated with estrogen (E2) for 2 d or E2 and medroxyprogesterone acetate (MPA) for 6 d. EEO cells were positive for the gland marker, FOXA2, and exhibited appropriate hormonal regulation of steroid hormone receptor expression. Real-time qPCR and bulk RNA-sequencing analysis revealed effects of hormone treatment on gene expression that recapitulated changes in proliferative and secretory phase endometrium. Single-cell RNA sequencing analysis revealed that several different epithelial cell types are present in the EEO whose proportion and gene expression changed with hormone treatment. The EEO model serves as an important platform for studying the physiology and pathology of the human endometrium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.