ArticlemSphere2019
Validating DNA Extraction Protocols for Bentonite Clay.
Article in mSphere, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Impact of Oxygen Release from Bentonite on Microbial Activity, Mineralogy, and Steel Corrosion.Environmental science & technology · 2025Article
- Seasonal dynamics of microbial diversity and functional potential in active sanitary landfill baseliner microbiomes.Biodegradation · 2025Article
- Validating digital polymerase chain reaction for 16S rRNA gene amplification from low biomass environmental samples.ISME communications · 2025Article
- Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite.Environmental microbiology reports · 2024Article
- Microbial hydrogen sinks in the sand-bentonite backfill material for the deep geological disposal of radioactive waste.Frontiers in microbiology · 2024Article
- N/S element transformation modulating lithospheric microbial communities by single-species manipulation.Microbiome · 2023Article
- RIAM: A Universal Accessible Protocol for the Isolation of High Purity DNA from Various Soils and Other Humic Substances.Methods and protocols · 2022Article
- Growth and Persistence of an Aerobic Microbial Community in Wyoming Bentonite MX-80 Despite AnoxicFrontiers in microbiology · 2022Article
- Fifteen shades of clay: distinct microbial community profiles obtained from bentonite samples by cultivation and direct nucleic acid extraction.Scientific reports · 2021Article
- Identifying Inorganic Turbidity in Water Samples as Potential Loss Factor During Nucleic Acid Extraction: Implications for Molecular Fecal Pollution Diagnostics and Source Tracking.Frontiers in microbiology · 2021Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bentonite clay is an integral component of the engineered barrier system of deep geological repositories (DGRs) that are planned for the long-term storage of high-level radioactive waste. Although nucleic acid extraction and analysis can provide powerful qualitative and quantitative data reflecting the presence, abundance, and functional potential of microorganisms within DGR materials, extraction of microbial DNA from bentonite clay is challenging due to the low biomass and adsorption of nucleic acids to the charged clay matrix. In this study, we used quantitative PCR, gel fingerprinting, and high-throughput sequencing of 16S rRNA gene amplicons to assess DNA extraction efficiency from natural MX-80 bentonite and the same material "spiked" with
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.