ArticleThe Plant cell2020
An Improved Recombineering Toolset for Plants.
Article in The Plant cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- EBSn, a Robust Synthetic Reporter for Monitoring Ethylene Responses in Plants.Plant biotechnology journal · 2026Article
- MONOPTEROS isoform MP11ir plays a role during somatic embryogenesis in Arabidopsis thaliana.Plant physiology · 2025Article
- DASH: a versatile and high-capacity gene stacking system for plant synthetic biology.Plant biotechnology journal · 2025Review
- A rapid and scalable approach to build synthetic repetitive hormone-responsive promoters.Plant biotechnology journal · 2024Article
- Clearing of Vascular Tissue in Arabidopsis thaliana for Reporter Analysis of Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Balanophora genomes display massively convergent evolution with other extreme holoparasites and provide novel insights into parasite-host interactions.Nature plants · 2023Article
- DELLA proteins positively regulate seed size in Arabidopsis.Development (Cambridge, England) · 2023Article
- Spatial and temporal localization of SPIRRIG and WAVE/SCAR reveal roles for these proteins in actin-mediated root hair development.The Plant cell · 2021Article
- A recombineering pipeline to clone large and complex genes in Chlamydomonas.The Plant cell · 2021Article
- A G protein-coupled receptor-like module regulates cellulose synthase secretion from the endomembrane system in Arabidopsis.Developmental cell · 2021Article
- Next Generation Cereal Crop Yield Enhancement: From Knowledge of Inflorescence Development to Practical Engineering by Genome Editing.International journal of molecular sciences · 2021Review
- The Diverse Salt-Stress Response of ArabidopsisPlants (Basel, Switzerland) · 2021Article
- Gibberellin-mediated RGA-LIKE1 degradation regulates embryo sac development in Arabidopsis.Journal of experimental botany · 2020Article
- Local auxin biosynthesis is required for root regeneration after wounding.Nature plants · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene functional studies often rely on the expression of a gene of interest as transcriptional and translational fusions with specialized tags. Ideally, this is done in the native chromosomal contexts to avoid potential misexpression artifacts. Although recent improvements in genome editing have made it possible to directly modify the target genes in their native chromosomal locations, classical transgenesis is still the preferred experimental approach chosen in most gene tagging studies because of its time efficiency and accessibility. We have developed a recombineering-based tagging system that brings together the convenience of the classical transgenic approaches and the high degree of confidence in the results obtained by direct chromosomal tagging using genome-editing strategies. These simple, scalable, customizable recombineering toolsets and protocols allow a variety of genetic modifications to be generated. In addition, we developed a highly efficient recombinase-mediated cassette exchange system to facilitate the transfer of the desired sequences from a bacterial artificial chromosome clone to a transformation-compatible binary vector, expanding the use of the recombineering approaches beyond Arabidopsis (
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