ArticleParasites & vectors2019
In silico selection of functionally important proteins from the mialome of Ornithodoros erraticus ticks and assessment of their protective efficacy as vaccine targets.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- In vivo evaluation of a cytochrome P450 gene from poultry red mite, Dermanyssus gallinae, as a vaccine antigen for chicks.Parasites & vectors · 2026Article
- Immunization with mRNA-LNPs EncodingPathogens (Basel, Switzerland) · 2025Article
- Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens.Parasites & vectors · 2024Article
- A multi-omics approach for understanding blood digestion dynamics in Ixodes scapularis and identification of anti-tick vaccine targets.Ticks and tick-borne diseases · 2024Article
- Cross-species immunoprotective antigens (subolesin, ferritin 2 and P0) provide protection against Rhipicephalus sanguineus sensu lato.Parasites & vectors · 2024Article
- Universal Tick Vaccines: Candidates and Remaining Challenges.Animals : an open access journal from MDPI · 2023Review
- Comparison of salivary gland and midgut microbiome in the soft ticksFrontiers in microbiology · 2023Article
- Early Transcriptional Changes in the Midgut ofMicroorganisms · 2022Article
- A proteomics informed by transcriptomics insight into the proteome of Ornithodoros erraticus adult tick saliva.Parasites & vectors · 2022Article
- Expounding the role of tick in Africa swine fever virus transmission and seeking effective prevention measures: A review.Frontiers in immunology · 2022Review
- Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens.Vaccines · 2021Article
- Review
- RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle.Parasites & vectors · 2021Article
- Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle.PLoS neglected tropical diseases · 2021Article
- Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation.Parasite (Paris, France) · 2021Article
- Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies.Vaccines · 2020Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundNew candidate protective antigens for tick vaccine development may be identified by selecting and testing antigen candidates that play key biological functions. After blood-feeding, tick midgut overexpresses proteins that play essential functions in tick survival and disease transmission. Herein, Ornithodoros erraticus midgut transcriptomic and proteomic data were examined in order to select functionally significant antigens upregulated after feeding to be tested as vaccine candidate antigens.
methodsTranscripts annotated as chitinases, tetraspanins, ribosomal protein P0 and secreted proteins/peptides were mined from the recently published O. erraticus midgut transcriptome and filtered in a second selection step using criteria based on upregulation after feeding, predicted antigenicity and expression in the midgut proteome. Five theoretical candidate antigens were selected, obtained as recombinant proteins and used to immunise rabbits: one chitinase (CHI), two tetraspanins (TSPs), the ribosomal protein P0 (RPP0) and one secreted protein PK-4 (PK4).
resultsRabbit vaccination with individual recombinant candidates induced strong humoral responses that mainly reduced nymph moulting and female reproduction, providing 30.2% (CHI), 56% (TSPs), 57.5% (RPP0) and 57.8% (PK4) protection to O. erraticus infestations and 19.6% (CHI), 11.1% (TSPs), 0% (RPP0) and 8.1% (PK4) cross-protection to infestations by the African tick Ornithodoros moubata. The joint vaccine efficacy of the candidates was assessed in a second vaccine trial reaching 66.3% protection to O. erraticus and 25.6% cross-protection to O. moubata.
conclusionsThese results (i) indicate that argasid chitinases and RPP0 are promising protective antigens, as has already been demonstrated for ixodid chitinases and RPP0, and could be included in vaccines targeting multiple tick species; (ii) reveal novel protective antigens tetraspanins and secreted protein PK-4, never tested before as protective antigens in ticks; and (iii) demonstrate that multi-antigenic vaccines increased vaccine efficacy compared with individual antigens. Lastly, our data emphasize the value of the tick midgut as a source of protective candidate antigens in argasids for tick control.
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