Evidence map›Paper›PMID 31666080›Full record

ReviewRespiratory research2019

Isolation and characterization of extracellular vesicles from Broncho-alveolar lavage fluid: a review and comparison of different methods.

Jonathan M Carnino, Heedoo Lee, Yang Jin

Abstract readComparative StudyReview
In one paragraph

Review in Respiratory research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
127citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

127 citing papers in PubMed, 1 synthesis or guideline pooled it.

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67 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jonathan M CarninoDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Boston University Medical Campus, 72 E Concord St. R304, Boston, MA, 02118, USA.
Heedoo LeeDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Boston University Medical Campus, 72 E Concord St. R304, Boston, MA, 02118, USA.
Yang JinDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Boston University Medical Campus, 72 E Concord St. R304, Boston, MA, 02118, USA. yjin1@bu.edu.

Funding

LncRNA regulates lung inflammationR01HL142758 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JIN, YANG · 2019 to 2022
$2.0M
Development of the RNA molecule-enriched, cell type-specific exosomesR33AI121644 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JIN, YANG · 2018 to 2020
$1.4M
Mechanistic insights of inflammation and organ failure after trauma or critical illnessR01GM127596 · NIGMS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JIN, YANG · 2018 to 2021
$1.3M
Mechanistic insights into the systemic inflammation and organ failure in sepsisR01GM111313 · NIGMS · BRIGHAM AND WOMEN'S HOSPITAL · PI JIN, YANG · 2014 to 2017
$1.3M
Development of the RNA molecule-enriched, cell type-specific exosomesR21AI121644 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI JIN, YANG · 2016 to 2017
$444k
National Institute of Allergy and Infectious Diseases R33AI121644NHLBI NIH HHS 1R01HL142758-01A1NHLBI NIH HHS R01 HL142758NIAID NIH HHS R21 AI121644NIAID NIH HHS R33 AI121644NIGMS NIH HHS R01 GM111313NIGMS NIH HHS R01GM111313NIGMS NIH HHS R01 GM127596NIGMS NIH HHS R01GM127596
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are cell-derived membranous vesicles secreted by cells into the extracellular space, which play a role in cell to cell communication. EVs are categorized into 3 groups depending on their size, surface marker, and method of release from the host cell. Recently, EVs have become of interest in the study of multiple disease etiologies and are believed to be potential biomarkers for many diseases. Multiple different methods have been developed to isolate EVs from different samples such as cell culture medium, serum, blood, and urine. Once isolated, EVs can be characterized by technology such as nanotracking analysis, dynamic light scattering, and nanoscale flow cytometry. In this review, we summarize the current methods of EV isolation, provide details into the three methods of EV characterization, and provide insight into which isolation approaches are most suitable for EV isolation from bronchoalveolar lavage fluid (BALF).

Indexed as

BiomarkersBronchoalveolar Lavage FluidChromatography, GelExtracellular VesiclesFlow CytometryHumansImmunoprecipitationMicrofluidic Analytical TechniquesUltracentrifugationBiomarkersApoptotic bodyBronchoalveolar lavage fluid (BALF)ExosomeExtracellular vesicle (EV)Microvesicle

Identifiers

PMID31666080
PMCPMC6822481

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.