ArticleBMC veterinary research2019
Detection of ALV p27 in cloacal swabs and virus isolation medium by sELISA.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 20 citations in OpenAlex.
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- Role of O-linked glycosylation modification on internalization and replication of avian leukosis virus subgroup J.Veterinary research · 2026Article
- Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh.Veterinary medicine and science · 2024Article
- Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application.Current issues in molecular biology · 2024Article
- Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023.Frontiers in microbiology · 2024Article
- Rapid detection of avian leukemia virus using CRISPR/Cas13a based lateral flow dipstick.Frontiers in veterinary science · 2024Article
- 3'UTR of ALV-J can affect viral replication through promoting transcription and mRNA nuclear export.Journal of virology · 2023Article
- Current Epidemiology and Co-Infections of Avian Immunosuppressive and Neoplastic Diseases in Chicken Flocks in Central China.Viruses · 2022Article
- Glycosylation of ALV-J Envelope Protein at Sites 17 and 193 Is Pivotal in the Virus Infection.Journal of virology · 2022Article
- Chicken hepatomegaly and splenomegaly associated with novel subgroup J avian leukosis virus infection.BMC veterinary research · 2022Article
- New rapid detection by using a constant temperature method for avian leukosis viruses.Frontiers in microbiology · 2022Article
- Novel mutation of avian leukosis virus subgroup J from Tibetan chickens.Poultry science · 2021Article
- Peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine.BMC veterinary research · 2021Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundAvian leukosis (AL), which is caused by avian leukosis virus (ALV), has led to substantial economic losses in the poultry industry. The kit used to detect all ALV-positive chickens in breeder flocks is very important for efficiently controlling AL. However, a new emerging ALV subtype is currently a severe challenge in the poultry industry.
resultsIn this paper, we compared different enzyme-linked immunosorbent assay (ELISA) kits for detecting p27 of ALV in the same batch of meconium samples. Different positive samples were further analyzed by PCR or virus isolation. The results showed that 36 positive samples among the 1812 chicken meconium samples could be detected by a sandwich ELISA (sELISA) kit, but only 17 positive samples could be identified by a commercial kit. To verify this result, cloacal swabs and viruses isolated from the positive chickens (2 days old) were used to detect the presence of p27. The results showed that the positive rate of p27 was 100% for the swabs and 40% for virus isolation. Surprisingly, PCR and sequence analysis revealed that the env gene of ALV in these positive samples belonged to the novel subgroup K (ALV-K).
conclusionThese data not only demonstrate the relatively high sensitivity of the sELISA kit but also highlight the challenge of controlling ALV-K.
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