ArticleThe FEBS journal2020
The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.
Article in The FEBS journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Photoswitchable intein for light control of covalent protein binding and cleavage.Nature communications · 2025Article
- A Unique THN Motif Is Critical for Enabling Efficient C-Terminal Traceless Cleavage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Conditional Split Inteins: Adaptable Tools for Programming Protein Functions.International journal of molecular sciences · 2025Review
- Ligation of multiple protein domains using orthogonal inteins with non-native splice junctions.Protein science : a publication of the Protein Society · 2024Article
- Rational Design of a Circularly Permuted Flavin-Based Fluorescent Protein.Chembiochem : a European journal of chemical biology · 2024Article
- Near-Infrared Optogenetic Module for Conditional Protein Splicing.Journal of molecular biology · 2023Article
- A cybergenetic framework for engineering intein-mediated integral feedback control systems.Nature communications · 2023Article
- Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.Frontiers in molecular biosciences · 2022Article
- Inteins as Drug Targets and Therapeutic Tools.Frontiers in molecular biosciences · 2022Review
- A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction.Nature communications · 2021Article
- Model-guided design of mammalian genetic programs.Science advances · 2021Article
- Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived fromBiochemistry · 2020Article
- In Cellulo Protein Semi-Synthesis from Endogenous and Exogenous Fragments Using the Ultra-Fast Split Gp41-1 Intein.Angewandte Chemie (International ed. in English) · 2020Article
- The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms.International journal of molecular sciences · 2020Article
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5 authors.
Funding
Abstract
Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 Å-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins. DATABASE: Structural data are available in the RCSB Protein Data Bank under the accession number 6QAZ.
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