Evidence map›Paper›PMID 31664956›Full record

ArticleBMC cancer2019

Exploring new potential role of DDB2 by host cell reactivation assay in human tumorigenic cells.

Elisabetta Bassi, Paola Perucca, Isabella Guardamagna, Ennio Prosperi, Lucia A Stivala, Ornella Cazzalini

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Elisabetta BassiDipartimento di Medicina Molecolare, Unità di Immunologia e Patologia generale, Università degli Studi di Pavia, Pavia, Italy.
Paola PeruccaDipartimento di Medicina Molecolare, Unità di Immunologia e Patologia generale, Università degli Studi di Pavia, Pavia, Italy.
Isabella GuardamagnaDipartimento di Medicina Molecolare, Unità di Immunologia e Patologia generale, Università degli Studi di Pavia, Pavia, Italy.
Ennio ProsperiIstituto di Genetica Molecolare (IGM) del CNR, Pavia, Italy. prosperi@igm.cnr.it.
Lucia A StivalaDipartimento di Medicina Molecolare, Unità di Immunologia e Patologia generale, Università degli Studi di Pavia, Pavia, Italy. luciaanna.stivala@unipv.it.
Ornella CazzaliniDipartimento di Medicina Molecolare, Unità di Immunologia e Patologia generale, Università degli Studi di Pavia, Pavia, Italy. ornella.cazzalini@unipv.it.ORCID http://orcid.org/0000-0003-2113-4158
University of Pavia · ITIstituto di Genetica Molecolare · IT

Funding

Associazione Italiana per la Ricerca sul Cancro 17041
6 · The paper itself

Abstract

backgroundThe Host Cell Reactivation assay (HCR) allows studying the DNA repair capability in different types of human cells. This assay was carried out to assess the ability in removing UV-lesions from DNA, thus verifying NER efficiency. Previously we have shown that DDB2, a protein involved in the Global Genome Repair, interacts directly with PCNA and, in human cells, the loss of this interaction affects DNA repair machinery. In addition, a mutant form unable to interact with PCNA (DDB2

methodsIn this work, we have investigated whether DDB2 protein may influence the repair of a UV-damaged DNA plasmid into the cellular environment by applying the HCR method. To this end, human kidney 293 stable clones, expressing DDB2

resultsThe results have shown that DDB2

conclusionThe HCR confirms itself as a very helpful approach to assess in the cellular context the effect of expressing mutant vs Wt NER proteins on the DNA damage response. Loss of interaction of DDB2 and PCNA affects negatively DNA repair efficiency.

Indexed as

DNA-Binding ProteinsDNA DamageDNA Excision Repair Protein ERCC-5DNA RepairEndonucleasesGenomic InstabilityGreen Fluorescent ProteinsHEK293 CellsHeLa CellsHumansLuminescent ProteinsMutant ProteinsMutationNuclear ProteinsPlasmidsProliferating Cell Nuclear AntigenDDB2 protein, humanDNA-Binding ProteinsDNA Excision Repair Protein ERCC-5EndonucleasesGreen Fluorescent ProteinsLuminescent ProteinsMutant ProteinsNuclear ProteinsPCNA protein, humanProliferating Cell Nuclear AntigenRed Fluorescent ProteinRNA Polymerase IITranscription FactorsDNA damaged binding protein 2DNA damage responseGlobal genome nucleotide excision repairRNA polymerase IIXeroderma Pigmentosum group G

Identifiers

PMID31664956
PMCPMC6819583
OpenAlexW2982537773

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.