ArticleChembiochem : a European journal of chemical biology2020
Phosphine-Activated Lysine Analogues for Fast Chemical Control of Protein Subcellular Localization and Protein SUMOylation.
Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
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- A Genetically Encoded Homocysteine Precursor to Probe Protein Active Sites and to Addict Escherichia coli to a Noncanonical Amino Acid Directly Involved in Catalysis.Angewandte Chemie (International ed. in English) · 2025Article
- Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag Protein.Bioconjugate chemistry · 2025Article
- Optogenetics with Atomic Precision─A Comprehensive Review of Optical Control of Protein Function through Genetic Code Expansion.Chemical reviews · 2025Review
- Investigating Protein Degradability through Site-Specific Ubiquitin Ligase Recruitment.bioRxiv : the preprint server for biology · 2024Article
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Cellular Site-Specific Incorporation of Noncanonical Amino Acids in Synthetic Biology.Chemical reviews · 2024Review
- Genetically Encoded Lysine Analogues with Differential Light Sensitivity for Activation of Protein Function.ChemPhotoChem · 2024Article
- Applications of genetic code expansion technology in eukaryotes.Protein & cell · 2024Review
- Direct Activation of Nucleobases with Small Molecules for the Conditional Control of Antisense Function.Angewandte Chemie (International ed. in English) · 2024Article
- Expanding the Genetic Code ofACS chemical biology · 2024Article
- Conditional Control of Universal CAR T Cells by Cleavable OFF-Switch Adaptors.ACS synthetic biology · 2023Article
- Conditional control of universal CAR T cells by cleavable OFF-switch adaptors.bioRxiv : the preprint server for biology · 2023Article
- Chemoselective Caging of Carboxyl Groups for On-Demand Protein Activation with Small Molecules.Angewandte Chemie (International ed. in English) · 2023Article
- Strategies for Conditional Regulation of Proteins.JACS Au · 2023Review
- Engineering Small Molecule Switches of Protein Function in Zebrafish Embryos.Journal of the American Chemical Society · 2023Article
- Small-Molecule Phosphine Activation of Protein Function in Zebrafish Embryos with an Expanded Genetic Code.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Efficient Amber SuppressionACS synthetic biology · 2022Article
- Small Molecule Control of Morpholino Antisense Oligonucleotide Function through Staudinger Reduction.Journal of the American Chemical Society · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Staudinger reduction and its variants have exceptional compatibility with live cells but can be limited by slow kinetics. Herein we report new small-molecule triggers that turn on proteins through a Staudinger reduction/self-immolation cascade with substantially improved kinetics and yields. We achieved this through site-specific incorporation of a new set of azidobenzyloxycarbonyl lysine derivatives in mammalian cells. This approach allowed us to activate proteins by adding a nontoxic, bioorthogonal phosphine trigger. We applied this methodology to control a post-translational modification (SUMOylation) in live cells, using native modification machinery. This work significantly improves the rate, yield, and tunability of the Staudinger reduction-based activation, paving the way for its application in other proteins and organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.